Alkaline Phosphatase Isoenzyme


“Everyone with COPD should be tested for A1AT”. That is the recommendation of both the W.H.O. and the American Lung Association and our new SPIFE Touch Alpha-1 Antitrypsin assay makes that much more feasible. SPIFE Touch A1AT allows identification of clinically relevant phenotypes on up to 40 samples per gel - twice the throughput of other assays using isoelectric focusing followed by immunofixation with anti-A1AT antisera. Separations are clear for greater ease in interpretation.

Alpha-1 Antitrypsin deficiency is the most common genetic risk factor for COPD as well as liver disease in children. Early diagnosis is essential. Lung damage from A1AT deficiency is irreversible, but treatment can slow progression. In the USA alone, there are 16 million people with COPD with millions more undiagnosed. Asymptomatic, heterozygous carriers could benefit from lifestyle modification with genetic counseling for family members.

SPIFE Touch A1AT Kit

Cat. No. / Item / Description

3432 –  SPIFE A1AT – 10 x 40 samples

Kit includes: 10 gels, Acid Violet Stain, Tris-Buffered Saline, Blotters, Wicks, Anode Solution, Cathode Solution, A1AT Antibody, Applicator Blades

A1AT Quality Control

Cat. No. / Item / Description

3435 –  SPIFE A1AT Controls (1 x 1mL each of MM, MS, MZ controls)

SPIFE A1AT Controls are lyophilized controls prepared from human serum for qualitative assessment of MM, MS and MZ phenotypes. Reconstituted control is stable for 14 days when stored at 2-8°C.

Supplies – SPIFE A1AT

Cat. No. / Item / Description

1347 –  SPIFE IEF Sample Tray
3360 –  SPIFE Disposable Cups (Deep Well)
3702 –  SPIFE IEF Tungsten Electrodes and Adapters
3706 –  SPIFE Reagent Spreaders

Additional Supplies and Equipment for SPIFE A1AT

1115 –  Gel Block Remover
3100 –  REP Prep Solution (1 x 250 mL)
3352 –  SPIFE Rigid Antisera Template IFE 15
3387 –  Applicator Blade Weights
4062 –  TITAN Gel Isoenzyme Incubation Chamber