SPIFE Assays

Split-Beta or 5-Band SPE

While some laboratorians prefer classic serum protein separations, others prefer a split-beta separation. With SPIFE Split-Beta gels, the proteins migrating in the beta region are split into two fractions, Beta 1 and Beta 2, between transferrin and C3 complement. In some instances, this may allow easier detection of small beta-migrating monoclonal gammopathies. Both the classic and split-beta formulations provide crisp, clear separation of the protein fractions.
- Samples are applied neat, no dilution hassles
- SPIFE’s application system is unaffected by triglycerides or large proteins
- Throughput of 1,400 samples in one 8-hour shift
- New QuickGel 10/20 sample gels plus SPIFE 20, 40, 60, 80 & 100 sample gels to suit any testing volume

Classic Serum Protein Kits

Cat. No. / Item / Description

3460 –  SPIFE SPE-60 (600 Profiles) – 10 x 60 samples
3461 –  SPIFE SPE-40 (400 Profiles) – 10 x 40 samples
3462 –  SPIFE SPE-20 (200 Profiles) – 10 x 20 samples
3405 –  QuickGel SPE Kit for SPIFE (5-Band) – 10 x 10/20 samples*

Kits include: 10 Gels, Acid Blue Stain, Citric Acid Destain, Blotters, Modified Applicator Blades.

For QuickGel SPE Kit for QuickGel Chamber, see Cat Nos. 3505 and 3505T.

Classic Urine and CSF Protein Kits

Cat. No. / Item / Description

3460U –  SPIFE SPE-60 for Urines – 10 x 60 samples
3461U –  SPIFE SPE-40 for Urines – 10 x 40 samples
3462U –  SPIFE SPE-20 for Urines – 10 x 20 samples

Kits include: 10 Gels, Acid Blue Stain, Citric Acid Destain, Blotters, Modified Applicator Blades.

Split Beta Serum Protein Kits

Cat. No. / Item / Description

3398 –  SPIFE Split Beta SPE-100 Kit– 10 x 100 samples
3399 –  SPIFE Split Beta SPE-80 Kit – 10 x 80 samples
3420 –  SPIFE Split Beta SPE-60 Kit – 10 x 60 samples
3421 –  SPIFE Split Beta SPE-40 Kit – 10 x 40 samples
3422 –  SPIFE Split Beta SPE-20 Kit – 10 x 20 samples
3350 –  QuickGel Split Beta SPE Kit – 10 x 10/20 samples*

Kits include: 10 Gels, Acid Blue Stain, Citric Acid Destain, Blotters, Modified Applicator Blades.

For QuickGel Split-Beta SPE Kit for QuickGel Chamber, see Cat Nos. 3550 and 3550T.

Split Beta Urine and CSF Protein Kits

Cat. No. / Item / Description

3398U –  SPIFE Split Beta SPE-100 Kit for Urines – 10 x 100 samples
3399U –  SPIFE Split Beta SPE-80 Kit for Urines – 10 x 80 samples
3420U –  SPIFE Split Beta SPE-60 Kit for Urines – 10 x 60 samples
3421U –  SPIFE Split Beta SPE-40 Kit for Urines – 10 x 40 samples
3422U –  SPIFE Split Beta SPE-20 Kit for Urines – 10 x 20 samples

Kits include: 10 Gels, Acid Blue Stain, Citric Acid Destain, Blotters, SPIFE Applicator Blades.

Serum Protein Controls

Cat. No. / Item / Description

3424 –  SPE Normal Control
3425 –  SPE Abnormal Control

These lyophilized controls are prepared from pooled human serum and are for use with the SPIFE SPE Vis and Split-Beta SPE kits listed above. Reconstituted controls are stable 5 days stored at 2 to 8- C. Each control is assayed for total protein, albumin, alpha1, alpha2, beta and gamma globulins.

Applicator Blades, Cups and Templates

for serum samples use

3451 – SPIFE Modified Applicator Blades, 30/pkg
3360 – SPIFE Dispo Sample Cups - Deep Well, 100/pkg

for Urine and csf samples use

3450 – SPIFE Applicator Blades, 30/pkg
3369 – SPIFE Dispo Sample Cups - Shallow Well, 100/pkg
3427 – SPIFE Urine/CSF Protein Accessory Kit (50 templates and blotters)
3380 – SPIFE Urine IFE Alignment Tray

NOTE: Urine and CSF specimens with insufficient volumes for automated sample application may be run using template application:

for quickgel split beta and spe kits use

3426 – QuickGel Accessory Kit (10 templates and blotters)

Additional Supplies and Equipment for SPIFE Protein Procedure:

REP Prep (Cat. No. 3100), Applicator Blade Weights (Cat. No. 3387), Gel Block Remover (Cat. No. 1115)

Additional Supplies and Equipment for QuickGel Protein Procedure:

Applicator Blade Weights (Cat. No. 3387), QuickGel Gel Block Remover (Cat. No. 1262), SPE Normal (Cat. No. 3424), SPE Abnormal (Cat. No. 3425), REP Prep (Cat. No. 3100), QuickGel Dispo Cup Tray (Cat. No. 3353), SPIFE QuickGel Electrode (Cat. No. 1111), SPIFE QuickGel Holder (Cat. No. 3358), SPIFE QuickGel Chamber Alignment Guide (Part No. 86541003)