Immunofixation

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Helena is a pioneer in the detection of monoclonal gammopathies by high resolution electrophoresis followed by immunoprecipitation, a technique called immunofixation. SPIFE ImmunoFix is an industry standard. Full-sized gels to accomodate from one to 15 profiles including IgG, IgA, IgM, K and λ, are available, with a maximum throughput of up to 210 profiles in a single 8-hour shift. Multichannel pipetting makes antisera application very efficient and antisera affinity and avidity are balanced for highest sensitivity with the least background blush.


SPIFE ImmunoFix Kits

Cat. No. / Item / Description

3408 –  SPIFE ImmunoFix-15 (150 Profiles) – 15 samples x 6 channels
3409 –  SPIFE ImmunoFix-9 (90 Profiles) – 9 samples x 6 channels
3401 –  SPIFE ImmunoFix-6 (60 Profiles) – 6 samples x 6 channels
3406 –  SPIFE ImmunoFix-3 (30 Profiles) – 3 samples x 6 channels
3351 –  QuickGel IFE Kit for SPIFE (20 Profiles) – 2 samples x 6 channels

SPIFE ImmunoFix Kits include: 10 Gels, Acid Violet Stain, Blotters, Tris-Buffered Saline, Citric Acid Destain, Applicator Blades, Blotter Combs, Applicator Swabs, Fixative, and 1 vial each of Antisera to IgG, IgA, IgM, Kappa and Lambda.

For QuickGel ImmunoFix Kit for QuickGel Chamber, see Cat No 3551T.


SPIFE ImmunoFix Gels and Antisera (Sold Separately)

Cat. No. / Item / Description

3413 –  SPIFE IFE Antisera Kit (3 mL ea Fixative, IgG, IgA, IgM, K, λ)
3465 –  SPIFE IFE Light Chain Antisera Kit (3 mL ea Fixative, K, λ)
9409 – IFE Antiserum to IgD – 1 x 1 mL
9410 –  IFE Antiserum to IgE – 1 x 1 mL
9412 –  IFE Free Kappa Antiserum – 1 x 1 mL
9413 –  IFE Free Lambda Antiserum – 1 x 1 mL
3466 –  SPIFE IFE-3 Gels (10 gels w/ consumables, no antisera)

3411 –  SPIFE IFE-6 Gels (10 gels w/ consumables, no antisera)
3469 –  SPIFE IFE-9 Gels (10 gels w/ consumables, no antisera)

ImmunoFix Quality Control

Cat. No. / Item / Description

9400 – ImmunoFix Contro Kit (1 x 0.5 mL each) IgG Kappa Control, IgA Lambda Control, IgM Control

ImmunoFix Controls are used to confirm antisera reactivity. After electrophoresis, the appropriate control is applied to a well at the end of each channel on the gel. Precipitin rings form around wells, indicating proper antigen/antibody reactions. Controls can also be treated as a patient sample to verify all phases of IFE procedure.

Supplies - SPIFE ImmunoFix - 9 and 15

Cat. No. / Item / Description

3363 –  SPIFE IFE-9/15 Dispo Sample Cups
3362 –  SPIFE IFE-15 Dispo Cup Tray
3378 –  SPIFE IFE-9 Dispo Cup Tray
3380 –  SPIFE Urine IFE-3/6/9 Alignment Guide
3407 –  SPIFE Urine IFE-15 Alignment Guide
3391 –  SPIFE Urine IFE-9/15 Accessories (50 templates and blotters)
3392 –  SPIFE IFE-9 Rigid Antisera Template
3352 –  SPIFE IFE-15 Rigid Antisera Template
3394 –  SPIFE IFE-9/15 Antisera Tray
3356 –  IFE 9/15 Tip Spacers for SPIFE Multi-Channel Pipette (3/pkg)
3355 –  Tips for SPIFE Multi-Channel Pipette (6 x 96/pkg)

Supplies - SPIFE ImmunoFix - 3 and 6

Cat. No. / Item / Description

3368 –  SPIFE IFE-3/6 Dispo Sample Cups
3377 –  SPIFE IFE-3/6 Dispo Cup Tray
3380 –  SPIFE Urine IFE-3/6/9 Alignment Guide
3390 –  SPIFE Urine IFE-3/6 Accessories (50 templates and blotters)
3395 –  SPIFE IFE-3 Rigid Antisera Template
3410 –  SPIFE IFE-6 Rigid Antisera Template
1119 –  SPIFE IFE-3/6 Antisera Tray
3349 –  IFE 6/3 Tip Spacers for SPIFE Multi-Channel Pipette (3/pkg)
3355 –  Tips for SPIFE Multi-Channel Pipette (6 x 96/pkg)

Supplies - QuickGel ImmunoFix on SPIFE

Cat. No. / Item / Description

3363 –  SPIFE IFE-9/15 Dispo Cups
3371 –  SPIFE QuickGel IFE Cup Tray
3372 –  SPIFE QuickGel IFE Rigid Antisera Template
1111 –  SPIFE QuickGel Electrodes
3358 –  SPIFE QuickGel Gel Holder
3373 –  SPIFE QuickGel IFE Chamber Alignment Guide
1262 –  QuickGel Gel Block Remover

Additional Supplies and Equipment for SPIFE IFE

Cat. No. / Item / Description

3100 –  REP Prep Solution (1 x 250 mL)
1122 –  SPIFE IFE Multi-Channel Pipette
1115 –  Gel Block Remover
3470 –  SPIFE IFE Weight


Download Booklet

an immunofixation tutorial

This guide is intended as a primer to the use and interpretation of immunofixation for characterizing monoclonal proteins (M-proteins) in human serum and urine. It contains examples of common and MGUS patterns, as well as those with complex, multiple proteins and light chains. Suggestions for follow-up testing and interpretive comments are included.