Helena can meet all your needs for identifying hemoglobin variants, taking full advantage of the automated sample application separation and staining of the SPIFE electrophoresis system. Both alkaline and acid methods are available.
Separations with SPIFE Alkaline Hemoglobin mimic classic cellulose acetate and are the front line for detecting hemoglobinopathies. Use of acid blue stain provides excellent sensitivity and linearity. Precise temperature control improves separation, resolving even problematic Hemoglobin H.
3415 – SPIFE Alk Hb - 20 Kit – 10 x 20 samples 3429 – QuickGel Alkaline Hemoglobin Kit for SPIFE – 10 samples
Kits include: 10 Gels, Acid Blue Stain, REP/SPIFE Hemolysate, Citric Acid Destain, Applicator Blades.
For QuickGel Alkaline Hemoglobin Kit for QuickGel Chamber, see Cat. No. 3529 and 3529T.
SPIFE Acid Hemoglobin is a much less cumbersome method than conventional citrate agar for characterizing hemoglobinopathies. The use of acid blue stain brings linearity not available with O-Tolidine or O-Dianisidine based systems. Finished gels are stable in storage and will not continue to darken.
3418 – SPIFE Acid Hemoglobin Kit – 10 x 20 samples 3419 – QuickGel Acid Hemoglobin Kit for SPIFE – 10 x 10/20 samples*
Kits include: 10 Gels, Acid Blue Stain, REP/SPIFE Hemolysate, Citric Acid, Destain, Applicator Blades.
*Additional SPIFE Applicator Blades (Cat. No. 3450) can be purchased to accommodate up to 20 samples per gel.
For QuickGel Acid Hemoglobin Kit for QuickGel Chamber, see Cat. No. 3519.
AFSC Hemo Control (Cat. No. 5331), AA2 Hemo Control (Cat. No. 5328), REP Prep (Cat. No. 3100), Gel Block Remover (Cat. No. 1115), Applicator Blade Weights (Cat. No. 3387), Disposable Sample Cups (Cat. No. 3369), SPIFE Disposable Cup Tray (Cat. No. 3370)
AFSC Hemo Control (Cat. No. 5331), AA 2 Hemo Control (Cat. No. 5328), REP Prep (Cat. No. 3100), SPIFE QuickGel Electrode (Cat. No. 1111), SPIFE QuickGel Holder (Cat. No. 3358), QuickGel Gel Block Remover (Cat. No. 1262),SPIFE QuickGel Chamber Alignment Guide (Part No. 86541003), Applicator Blade Weights (Cat. No. 3387), Disposable Sample Cups (Cat. No. 3369), QuickGel Dispo Cup Tray (Cat. No. 3353), Chamber Cover Part No. 8JP34012)