Alkaline Phosphatase Isoenzyme

Alkaline Phosphatase Isoenzyme

Alkaline phosphatase (ALP) isoenzymes found in human serum originate from several sources with the greatest activity found in bone, liver, intestine, and the placenta. Tissue sources of elevated alkaline phosphatase in serum can be determined by identifying the isoenzyme. The isoenzymes differ in their physicochemical and electrophoretic properties, allowing identification. In addition to liver, bone, intestinal and placental isoenzymes, macrohepatic, Regan, PA, Nagao, and renal isoenzymes have been identified. These isoenzymes can be identified using the SPIFE analyzer.

SPIFE ALP agarose gels have a unique additive in the gel matrix to ensure separation of macrohepatic from liver and bone fractions. Sample pre-treatment with neuraminidase provides greater separation. Up to 40 samples per gel can be assayed with less than 5 minutes hands-on time.

SPIFE Alkaline Phosphatase Isoenzyme Kits

Cat. No. / Item / Description

3345 –  SPIFE ALP-20 Kit – 10 x 20 samples/gel
3346 –  SPIFE ALP-40 Kit – 10 x 40 samples/gel

Kits include: 10 Gels, ALP Reagent, ALP Diluent, Blotters, Citric Acid Destain, Applicator Blades.

Alkaline Phosphatase Isoenzyme Control

Cat. No. / Item / Description

5104 –  Gel ALP Isoenzyme Control– 1 x 2 mL

This control is for use as a qualitative and/or quantitative control to aid in the identification of alkaline phosphatase isoenzymes by agarose gel electrophoresis. It is prepared from pooled human serum and contains liver and bone isoenzyme bands. The control is a stabilized liquid.

SPIFE ALP Separation Enhancer

Cat. No. / Item / Description

3348 –  SPIFE ALP Separation Enhancer – 1 x 4 mL

The neuraminidase in the Separation Enhancer causes a greater reduction in mobility of the bone enzyme than the liver isoenzyme upon electrophoresis. 50 μL of patient sample is pre-treated with 10 μL of separation enhancer within 10 minutes of electrophoresis, to provide greater separation of bone and liver isoenzymes.

Additional Supplies and Equipment for SPIFE ALP Procedure:

Gel Block Remover (Cat. No. 1115), SPIFE Reagent Spreader (Cat. No. 3386), REP Prep (Cat. No. 3100), SPIFE 20-100 Dispo Cup Tray (Cat. No. 3366), and SPIFE Dispo Cups (Deep Well)- (Cat. No. 3360).