IgG Isoelectric Focusing
What is Oligoclonal Banding?
When there is a restricted humoral immune response, oligoclonal bands can be detected in the patient’s cerebrospinal fluid. The concentration of individual bands may be as low as 2% of the total CSF IgG but can be seen against the background of polyclonal IgG present through filtration from the blood. Due to this filtration, it is important to rule out oligoclonal IgG from the blood by running parallel serum samples against CSF samples. The presence of oligoclonal banding in CSF but not in serum is indicative of MS.
SPIFE Touch IgG Isoelectric Focusing
The SPIFE IgG IEF technique is the “gold standard” for determining intrathecal IgG synthesis. This method uses highly sensitive immunoblotting resulting in a 95% sensitivity level. The concentration of individual bands may be as low as 2% of the total CSF IgG but can be seen even while the total CSF IgG concentration is within the reference range.
Isoelectric focusing is considered twice as sensitive as the IgG index and gives superior resolution over standard electrophoresis.
SPIFE IgG Isoelectric Focusing for CSF and Serum
Cat. No. / Item / Description
3389 – SPIFE IgG IEF Kit – 10 x 5 profiles per gel*
3385 – SPIFE IgG IEF 20 Kit – 10 x 10 profiles per gel*
Kits include: Anode Solution, Cathode Solution, Blocking Agent, Acetate Buffer Concentrate, Chromogen, IgG IEF Antibody, Transfer Membranes, Blotters, Wicks, Sample Templates.
3384 – SPIFE IgG IEF Chromogen (5 vials)
High Resolution Protein Marker
Cat. No. / Item / Description
5141 – High Resolution Protein Marker – 1 x 0.2 mL
For use as a qualitative marker with the SPIFE and TITAN GEL systems, the marker simulates oligoclonal banding in CSF and serum. This provides assurance that the necessary degree of resolution has been achieved, and offers a visual reference for differentiating oligoclonal bands. The marker is a stabilized liquid prepared from human serum.
Additional Supplies and Equipment for SPIFE IgG IEF:
REP Prep (Cat. No. 3100), SPIFE IgG IEF Electrodes (Cat. No. 3383), SPIFE IgG IEF Square Electrodes (Cat. No. 3703), SPIFE IEF Adapter and Electrodes (Cat. No. 3704), Development Weight (Cat. No. 5014), Staining Dish (Cat. No. 4061)