IEP is a reliable and accurate method for routine serum and urine protein evaluations, detecting both structural abnormalities and concentration changes. It has been widely used in identification of monoclonal gammopathies. Immunoelectrophoresis (IEP) combines two techniques, electrophoresis and immunodiffusion. In this two-part procedure, proteins are first separated by electrophoresis. Then antisera, complementary to the proteins under study, are applied to the gel and allowed to diffuse. When a favorable antigen to antibody ratio exists, a precipitin arc forms on the gel.
Both thick and thin gels are available for performing immunoelectrophoresis. The thick agarose, on a rigid plastic backing for ease of handling, requires a larger volume of antisera and patient samples, and forms a dense precipitin arc easily interpreted and photographed without staining the gel. The thin agarose gel, on a flexible plastic backing, provides economical use of antisera and lends itself to staining and drying of the gel for a permanent record.
|3049||TITAN GEL IEP Stain, 1 x 10 g|
The stain is a Coomassie Brilliant Blue Stain in powder form designed especially for use with the TITAN GEL IEPlate Kit (Cat. No. 3047). Stain is dissolved in 1 L mixture of water, ethanol, and glacial acetic acid.
Remember:- IEP Sponge Wicks - Long (Cat. No. 9015), Dialamatic 1-10 μL Microdispenser (Cat. No. 6210), Electra B1 Buffer (cat. No. 5016),- Normal Human Serum Control (Cat. No. 9010),- Albumin Marker (Cat. No. 9011).
|9009||Double Diffusion Plates, 10 gels/pkg|
Gels contain 15 wells in three, 5-well configurations. Useful in determining antisera titer, antigen/antibody ratio, checking purity of antigen preparations, etc.
|9010||Normal Human Serum Control, 1 x 2 mL|
|9011||Albumin Marker, 1 x 2 mL|
The control is a stabilized liquid prepared from pooled normal human serum for use as a normal control serum for immunoelectrophoresis procedures. Albumin Marker is bromphenol blue which binds to albumin. The marker is added to the Normal Human Serum Control so the control can be used as a migration marker in immunoelectrophoresis procedures.
Remember:- IEP Sponge Wicks - Long (Cat. No. 9015),- Electra B1 Buffer (Cat. No. 5016).