Immunofixation electrophoresis (IFE) is characterized by its enhanced sensitivity, ease of interpretation, quick test results, and excellent resolution. IFE is a two stage procedure using agarose protein electrophoresis first, then immunoprecipitation. The specimen may be serum, urine or CSF. There are many applications for IFE but the primary use is characterization of paraproteins. Pre-cut wells on each antisera lane allow use of TITAN GEL ImmunoFix Controls to provide verification of antisera reactivity on each patient gel.
3046 – TITAN GEL ImmunoFix Kit – 10 x 6 channels/gel
3046A – TITAN GEL ImmunoFix Gels – 30 gels, no antisera
Each channel of the ImmunoFix-6 gel has pre-cut application wells for TITAN GEL IFE Controls (Cat. No. 9400) to confirm antisera reactivty. Kits include: 10 Gels, Acid Blue Stain, IFE Buffer, Sample Templates, Antisera Templates, Protein Fixative, Blotters, and 1 x 0.75 mL vial each of Antisera to IgG, IgA, IgM, Kappa and Lambda.
3067 – TITAN GEL ImmunoFix-Plus – 10 x 12 channels/gel
Kits include: 10 Gels, Acid Blue Stain, IFE Buffer, Blotters, Sample Templates, Antisera Templates.
TITAN GEL ImmunoFix-Plus allows testing of two samples simultaneously. Antisera are purchased separately specific to your needs. Each channel of the ImmunoFix-Plus gel has pre-cut application wells for TITAN GEL IFE Controls (Cat. No. 9400) to confirm antisera reactivty.
3068 – ImmunoFix - Plus Antisera Kit – 1 x 0.75 mL vial each of Fixative, IgG, IgA, IgM, Kappa and Lambda
3151 – IFE Antisera Kit II – 1 x 3 mL vial each of Fixative, IgG, IgA, IgM, Kappa and Lambda
3069 – ImmunoFix-Plus Light Chain Antisera Kit – 2 x .075 mL vial each of Fixative, Kappa, Lambda
9409 – TITAN GEL IFE Antiserum to IgD – 1 x 1 mL
9410 – TITAN GEL IFE Antiserum to IgE – 1 x 1 mL
9412 – IFE Antisera to Free Kappa Light Chains – 1 x 1 mL
9413 – IFE Antisera to Free Lambda Light Chains – 1 x 1 mL
Helena provides premium quality antisera, carefully balanced for affinity and avidity for the greatest sensitivity the least amount of background blush. Kits for typical immunofixation profiles are available as well as specialty antisera. IgD and IgE antisera should be used if a reaction occurs with kappa or lambda without a reaction with IgG, A or M heavy chains. The presence of light chains not attached to the heavy chain portion of the immunoglobulin molecule can be confirmed with TITAN GEL IFE Free Kappa and Free Lambda Antisera.
9400 – ImmunoFix Control Kit
1 x 0.5 mL IgG Kappa Control – 1 x 0.5 mL IgA Lambda Control – 1 x 0.5 mL IgM Control
TITAN GEL ImmunoFix controls are used to confirm antisera reactivity on each patient gel. After electrophoresis, the appropriate control is applied to a well at the end of each channel on the gel. When antisera applied to the channels comes in contact with the control serum, an antigen/antibody reaction occurs, causing a precipitin ring to form around each well on the finished gel. To verify all phases of the procedure, the control can be treated as a patient sample and taken through every step of the procedure. The laboratory may elect to perform this quality control procedure on one gel from each lot of kits received.
IEP is a reliable and accurate method for routine serum and urine protein evaluations, detecting both structural abnormalities and concentration changes. It has been widely used in identification of monoclonal gammopathies. Immunoelectrophoresis (IEP) combines two techniques, electrophoresis and immunodiffusion.
3047 – TITAN GEL IEPlate Kit – 10 IEPlates plus blotters
TITAN GEL IEPlates provide fast, economical immunoelectrophoresis. The thin gel uses a reduced sample volume of 2 mL and antisera volume of 25 mL which means tremendous cost savings. Each gel has eight wells and seven troughs to accommodate a wide variety of antisera profiles. After an 18-hour incubation, non-precipitated proteins are washed from the gel. The gel can then be stained, destained and dried within one hour. The printed plastic backing makes sample and control identification easy and includes a preprinted scale for measuring migration distance.
For Titan IV Immunoelectrophoresis, see Cat. No. 9050 and Cat No. 9061.
9231 – Antisera to Human IgA, Heavy Chain Specific – 1 x 2 mL
9232 – Antisera to Human IgG, Heavy Chain Specific – 1 x 2 mL
9233 – Antisera to Human Whole Serum – 1 x 2 mL
9234 – Antisera to Human IgM, Heavy Chain Specific – 1 x 2 mL
9236 – Antisera to Human (IgG, A, M) Trivalent, Heavy Chain Specific – 1 x 2 mL
9249 – Antisera to Human IgD, Heavy Chain Specific – 1 x 2 mL
9250 – Antisera to Human IgE, Heavy Chain Specific – 1 x 2 mL
9251 – Antisera to Human (IgG, A, M, D, E) Pentavalent, Heavy Chain Specific – 1 x 2 mL
9257 – Antisera to Human Lambda, LIght Chain Specific – 1 x 2 mL
9262 – Antisera to Human Kappa, Light Chain Specific – 1 x 2 mL
Helena's antisera are produced in goats or sheep.
3049 – TITAN GEL IEP Stain – 1 x 10 g
The stain is a Coomassie Brilliant Blue Stain in powder form designed especially for use with the TITAN GEL IEPlate Kit (Cat. No. 3047). Stain is dissolved in 1 L mixture of water, ethanol, and glacial acetic acid.
9010 – Normal Human Serum Control – 1 x 2 mL
9011 – Albumin Marker – 1 x 2 mL
The control is a stabilized liquid prepared from pooled normal human serum for use as a normal control serum for immunoelectrophoresis procedures. Albumin Marker is bromphenol blue which binds to albumin. The marker is added to the Normal Human Serum Control so the control can be used as a migration marker in immunoelectrophoresis procedures.
The key roles which serum proteins play in bodily functions and the relative ease of separating them by electrophoresis, make their determination a valuable diagnostic tool as well as a way to monitor clinical progress. Total serum protein levels and serum protein electrophoresis should be included in every laboratory work-up on a patient. There is no other single procedure, or even group of procedures, that is capable of detecting so broad a spectrum of disease states or is as indicative of the degree of health as serum protein electrophoresis.
3041 – TITAN GEL Serum Protein Kit – 10 x 10 samples/gel
Kits include: 10 Gels, Serum Protein Buffer, Amido Black Stain, Templates, Blotters.
5136 – SPE Control – 1 x 2 mL
The control is for qualitative and/or quantitative use in agarose serum protein electrophoresis. It is a stabilized liquid prepared from human serum and assayed for total protein, albumin, alpha1, alpha2, beta and gamma globulins.
For controls for SPIFE Serum Protein assays, order Cat. No. 3424 or 3425.
Fifteen or so proteins can be visualized by use of high resolution, high sensitivity agarose gel electrophoresis of serum.The superior resolution and sensitivity of the procedure separates and detects proteins with similar electrophoretic mobilities and those present in low concentrations that may be invisible on other methods.
3040 – TITAN GEL Hi Res Protein Kit 10 x 7 samples/gel
Kits include: 10 Gels, HR Buffer, HR Coomassie Brilliant Blue Stain, Blotters, Wicks, Templates.
See also Cat. No. 3048 - Amido Black Protein Stain (an optional alternative to Coomassie Brilliant Blue Stain)
5141 – High Resolution Protein Marker 1 x 0.2 mL
A qualitative marker for use with the TITAN GEL, REP and SPIFE High Resolution Protein Systems. The marker exhibits multiple bands and simulates oligoclonal banding in CSF and serum. It provides assurance that sufficient resolution has been achieved and is a visual reference for differentiating oligoclonal bands. The marker is a stabilized liquid prepared from human serum.
3058 – TITAN GEL Hi Res Alk Phos Isoenzyme Kit – 10 x 8 samples/gel
Kits include: 10 Gels, ALP Buffer, ALP Separation Enhancer, ALP Reagent, ALP Diluent, Blotter, Wicks, Templates.
The TITAN GEL high resolution agarose electrophoresis method for separation of alkaline phosphatase isoenzymes provides excellent resolution. Neuraminidase is used to enhance separation of bone and liver fractions. A unique additive to the gel matrix ensures separation of the macrohepatic fraction from bone and liver. The excellent sensitivity of the system allows detection and separation of ALP isoenzymes even in samples with low activity. Up to eight samples may be applied per gel.
5104 – Gel ALP Isoenzyme Control – 1 x 2 mL
This control is for use as a qualitative and/or quantitative control to aid in the identification of alkaline phosphatase isoenzymes by agarose gel electrophoresis. It is prepared from pooled human serum and contains liver and bone isoenzyme bands. The control is a stabilized liquid and may also be used with SPIFE assays.