hg Electrophoresis Supplies and Accessories

Titan Plus Power Supply


Cat. No. / Item / Description

1504 –  Titan Plus Power Supply – 110V
1505 –  Titan Plus Power Supply – 220V


Titan Plus is a completely solid state unit. Digital voltage and current display provides greater accuracy in voltage selection. Synchronous electrophoresis timer assures precise control; timer automatically shuts off current and signals end of run. For use with cellulose acetate, agarose and agar. Recommended for use with Quick Gel Chamber, (Cat. No. 1284) and TITAN GEL Chamber, (Cat. No. 4063).

Voltage:
0-650 VDC. Digital display. Accuracy - 3V.
Current:
Up to 120 mA. Digital display. Accuracy - 2 mA.
Chamber Requirements:
Accepts all standard electrophoresis chambers
Timer:
Synchronous, electrical. Up to 60 minutes automatic timing. Audiovisual signal indicates end of run. "Hold" allows for continuous operation beyond 60 minutes.
Safety Feature:
Fuse holder: 110V-1A; 220V-.5A, 3 prong plug, grounded chassis
Output Jacks:
Two sets labeled three ways: + and -, Red and Black; Positive and Negative. Standard banana jack spacing.
Dimensions:
5" H x 10" W x 9.5" D (12.8 x 25.5 x 24.2 cm)
Weight:
11 lbs. (4.95 kg)


Electrophoresis Chambers and Accessories


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TITAN GEL Chamber

Cat. No. / Item / Description

4063 –  TITAN GEL Chamber

The TITAN GEL Chamber is for Titan III cellulose acetate, TITAN GEL Agarose, Titan IV citrate agar and IEP gels. Disposable paper wicks or sponges allow use with electrophoresis media supported by a rigid, flat base up to 6" x 9". Unique interlock system breaks electrical circuit when chamber lid is removed.

Paper and Sponge Wicks

Cat. No. / Item / Description

5081 –  Zip Zone Chamber Wicks – 500/pkg
9014 –  Sponge Wicks - Short – 4 short sponges/pkg
9015 –  Sponge Wicks - Long – 2 long sponges/pkg

Zip Zone Chamber Wicks are disposable paper wicks used with the Titan III cellulose acetate system to provide buffer flow during electrophoresis. Sponge Wicks provide sufficient buffer flow during agar and agarose techniques such as IEP and citrate hemoglobin electrophoresis.

Blotters

Titan Blotter Pads

Cat. No. / Item / Description

5034 –  Blotter Pads – 76 x 102 mm – 100/box
5037 –  Blotter Pads – 89 x 108 mm – 100/box
5098 –  Blotter Pads – 203 x 241 mm – 25/pkg

These soft, absorbent paper blotters are used to remove excess buffer or reagent from cellulose acetate membranes or agarose gels.
Note: Blotters for Helena agarose procedures are packaged in each gel kit.

Buffers

Electra, Citrate and Supre-Heme Buffers

Cat. No. / Item / Description

5016 –  Electra B1 Buffer Barbital/Sodium Barbital/Na Azide – 10 x 13.1 g
5121 –  Citrate Buffer Sodium Citrate/Citric Acid – 10 x 15.5 g
5802 –  Supre-Heme Buffer Tris-EDTA/Boric Acid – 10 x 14.6 g
5805 –  Electra HR Buffer Tris-Barbital/Sodium Barbital - 10 x 18 g

Note: Appropriate buffers for- SPIFE, REP and TITAN GEL Agarose kits are packaged as part of each kit.

Stains, Clearing Solutions

Cat. No. / Item / Description

3048 –  Amido Black Protein Stain – 1 x 2.5 g

Used as an alternative to Coomassie Brilliant Blue in the TITAN GEL High Res Protein procedure and for other procedures requiring a good basic protein stain. Dissolve stain in 450 mL methanol, 450 mL deionized water and 100 mL glacial acetic acid.

Cat. No. / Item / Description

3049 –  TITAN GEL IEP Stain – 1 x 10 g

Coomassie Brilliant Blue stain for use with the TITAN GEL IEPlate Kit (Cat. No. 3047). Dissolve in 450 mL ethanol, 450 mL deionized water and 100 mL glacial acetic acid.

Cat. No. / Item / Description

5322 –  Lipoprotein Stain – 1 x 2.8 g

Fat Red 7B stain for use with Titan III-Lipo cellulose acetate. Dissolve stain in 1000 mL methanol.

Cat. No. / Item / Description

5526 –  Ponceau S Stain – 1 x 105 g

Multi-purpose protein stain, especially useful for serum proteins and alkaline hemoglobins on cellulose acetate. Dissolve in 1000 mL deionized water.

Cat. No. / Item / Description

5036 –  o-Dianisidine – 1 x 2 g

Hemoglobin-specific stain for Titan IV Citrate Agar. Dissolve stain in 1000 mL methanol.

Cat. No. / Item / Description

5041 –  o-Tolidine – 1 x 2 g

Hemoglobin-specific stain yields blue-colored bands with Titan IV Citrate Agar. Not recommended when 5% acetic acid wash is used. Dissolve stain in 1000 mL methanol.

Cat. No. / Item / Description

5005 –  Clear Aid – 1 x 250 mL

Clearing solution additive that prevents cellulose acetate from shrinking during the clearing step. Add this high molecular weight elastomer as directed in the cellulose acetate procedures for proteins and alkaline hemoglobins.

Cat. No. / Item / Description

4950 –  PermaClear – 1 x 1 L

A substitute for the clearing solution prepared with Clear Aid. Not recommended for use with AVES or AVES 2. Dilute PermaClear with deionized water (55 mL PermaClear/45 mL water).

Buffer and Stain Accessories

Bufferizer

Cat. No. / Item / Description

5093 –  Bufferizer – 1/pkg

Bufferizer is used to uniformly soak cellulose acetate membranes prior to electrophoresis. To use, place up to 12 dry membranes in a carrying rack; set rack in Bufferizer bottom; place Bufferizer top over bottom; pour buffer into top. Buffer gradually drips through a small hole in the top, allowing a uniform liquid front to move up the dry membranes. Eliminates air bubbles caused by uneven soaking.


Staining Sets and Carrying Racks

Cat. No. / Item / Description

5122 –  1000 mL Staining Set, 7 trays with lids – 1 rack
5110 –  Titan III Carrying Rack – 1/pkg

Staining set consists of 7 plastic trays with lids and a carrying rack for staining and soaking of cellulose acetate membranes. Trays hold 1000 mL of liquid. Carrying rack is designed to hold up to twelve 60 x 76 mm or 94 x 76 mm Titan III cellulose acetate membranes. One rack is included with the staining set; rack is also available separately.

Staining Dishes

Cat. No. / Item / Description

4061 –  Staining Dish (175 x 102 x 50 mm) – 1/pkg
1362 –  REP Gel Staining Dish (216 x 168 x 19 mm)– 10/pkg

The Staining Dish (Cat No 4061) and the larger REP Gel Staining Dish (Cat No 1362) may be used for cellulose acetate and agarose electrophoresis assays requiring staining.

Incubation Chamber and Development Weight

Incubation Chamber

Cat. No. / Item / Description

4062 –  TITAN GEL Isoenzyme Incubation Chamber – 1/pkg

The TITAN GEL Isoenzyme Incubation Chamber is 171 x 89 x 6 mm and holds one gel. It maintains adequate humidity during the incubation process when running TITAN GEL ImmunoFix procedures.

Development Weight

Cat. No. / Item / Description

5014 –  Development Weight, 102 x 108 mm – 1/pkg

The Development Weight can be used to press agarose gels. The weight also provides a constant surface temperature for incubating cellulose acetate membranes. Membranes are sandwiched between a prewarmed weight and a glass slide, then placed in incubator.

Sample Application Devices

Applicators

Super Z, Super Z-12 and Super CK are used with Titan III cellulose acetate. Tips are photo-etched stainless steel and independently suspended to eliminate application artifacts. The only pressure exerted on the cellulose acetate is the weight of the tip. Super Z and Z-12 applicators hold 0.25 μL of sample; Super CK applicator holds 0.5 μL. Super Z and Super CK applicators apply 8 samples simultaneously, Super Z-12 applies 12 samples.

Kits include the applicator, two sample well plates, aligning base and a bottle of Zip Zone Prep.

Cat. No. / Item / Description
Applicator Kit* Applicator Well Plate Aligning Base Tips
-   1/pkg 2/pkg 1/pkg 2/pkg
Super Z 4088 4084 4085 4086 4087
Super Z-12 4093 4090 4096 4094 4087
Super CK - - - 4094 -


Zip Zone and Titan IV Applicator System

Cat. No. / Item / Description

4080 –  Zip Zone Applicator – 1/pkg
4081 –  Zip Zone Sample Well Plate – 1/pkg
4082 –  Zip Zone Aligning Base – 1/pkg
4083 –  Titan IV Aligning Base – 1/pkg
5090 –  Zip Zone Prep – 1 x 60 mL

Zip Zone Applicator System is used with Titan IV Citrate Agar Hemoglobin Electrophoresis. Plastic tips apply samples uniformly without cutting agar. Titan IV Aligning Base allows two off-center applications for a total of 16 samples. Use Zip Zone Prep to clean applicator tips.

5 μL Microdispenser

Cat. No. / Item / Description

6008 –  5 μL Microdispenser – 1 dispenser – 100 bores
6010 –  Ziptrol Glass Bore Tubes – 100/pkg

Microdispenser features precision stainless steel plunger and disposable glass bores. Aluminum "collars" allow dispensing from 1 to 5 μL, in 1 μL increments.

Dialamatic Microdispenser

Cat. No. / Item / Description

6210 –  10 μL Microdispenser – 1 mL increments
6211 –  10 μL Dialamatic Tubes – 100/pkg
6225 –  25 μL Microdispenser – 1 mL increments
6226 –  25 μL Dialamatic Tubes – 100/pkg
6250 –  50 μL Microdispenser – 2 mL increments
6251 –  50 μL Dialamatic Tubes – 100/pkg
6276 –  100 μL Microdispenser – 100/pkg
6228 –  Parts Kit for 25 μL Microdispenser – 3 Teflon tips, 1 plunger, 2 o-rings, 1 collet, 1 coil spring & 1 spring guide
6253 –  Parts Kit for 50 μL Microdispenser – 3 Teflon tips, 1 plunger, 2 o-rings, 1 collet, 1 coil spring & 1 spring guide

Dialamatic Microdispensers feature: sample preset, stainless steel plunger (10 mL unit), replaceable plunger tips (25, 50, and 100 mL units), precision glass disposable bores, - 1% volumetric tolerance.

Miscellaneous Electrophoresis Supplies

Titan and TITAN GEL Plastic Envelopes

Cat. No. / Item / Description

5052 – Titan Envelopes – 121 x 64 mm, 200/pkg
5053 – Titan Envelopes – 115 x 102 mm, 100/pkg
5054 – TITAN GEL Envelopes – 86 x 156 mm, 100/pkg

These clear plastic envelopes, with convenient overlapping sleeve, enable you to store completed electrophoresis membranes and gels without scratching or tearing. They afford ideal protection for both completed TITAN III cellulose acetate and TITAN GEL agarose gels.

Helena Marker

Cat. No. / Item / Description

5000 – Helena Marker – 1/pkg

The Helena Marker can be used to write identifying information on the support side of cellulose acetate membranes and agarose gels. Will not wash off in most solvents.

Helena Glue Stick

Cat. No. / Item / Description

5002 – Glue Stick – 1/pkg

The Glue Stick is an easy-to-use adhesive for mounting densitometric tracings on the report forms.

Identification Labels

Cat. No. / Item / Description

5006 – Titan ID Labels – 121 x 64 mm, 200/pkg

Titan ID Labels are convenient, pressure-sensitive identification labels for Titan III membranes. Simply press label onto edge of membrane or Titan Plastic Envelope to provide a paper surface on which to identify the sample.

CliniScan 2, CliniScan 3, EDC Accessories, Parts and Service

Cat. No. / Item / Description

1044 – DZA Replacement Lamp for REP, EDC, CliniScan 2
1047 – Optical Density Step Tablet – 1/pkg

Neutral Density Densitometer Controls

Cat. No. / Item / Description

1031 – Neutral Density Densitometer Control for QuickScan

The NDDC can be used to verify densitometer accuracy. The NDDC provides a test scan with known values for 5 fractions. To use, scan the NDDC as you would an electrophoresis pattern. The values obtained should correlate to the printed values for each fraction.