V8 capillary


Total Automation for IFEs and SPEs

Yes, you really can just load the sample tubes, press go, and return for your printed IFE and SPE results! SPIFE 4000 eliminates ALL manual steps for these assays - there’s no sample application, no stains or antisera to add, no gel transfers, no dilutions to perform, no gel blocks to remove, no tech-to-tech variability - just load and walk away. A full day’s mixed run of SPEs and IFEs takes about 30 minutes of tech time for set up, then frees the tech to perform other tasks. In a single 8-hour shift, SPIFE 4000 can turn 100 IFEs or 756 SPEs. With SPIFE 4000’s hands-off approach, a laboratory could reasonably expect to be able to run 24 hours per day 7 days a week.


SPIFE 4000 Features

Auto Sampling for SPE and IFE
- Liquid level sensing
– Auto dilutions for immunofixation
- Positive ID from tube to gel to final report

Auto Applicator and Electrophoresis
- Automatic application of samples to gels
– Application system unaffected by triglycerides or large proteins
- Peltier-driven temperature controlled chamber

Auto Immunofixation
- Color-coded antisera stored on-board, ready to use
– Antisera applied through a rigid template to eliminate crossover
- Auto press and blot to assure clear background

Auto Staining and Drying
- On-board staining with no gel transfers
– Auto destain and wash for clear backgrounds
- Automated drying and transfer to scanner

Integrated Auto Staining
- Automatic setting of scan parameters
– Full editing and interpretation capabilities
- Final results can be printed and/or exported to LIS

Powerful User Interface
- Bi-directional LIS interface
– System provides status of each gel in process
- On-screen system alerts


Cat. No. Item
1620 SPIFE 4000, 110V
1621 SPIFE 4000, 220V
1625 SPIFE 4000 Replacement Electrodes
1630 SPIFE 4000 Cassettes (10/pkg)
1635 SPIFE 4000 Rigid Antisera Template
1636 SPIFE 4000 Serum Separator Sleeves, 28/pkg
2304 SPIFE 4000 IFE Antisera Pipette Tips, 96/pkg
2305 SPIFE 4000 IFE Sample Trays, 10/pkg
2307 SPIFE 4000 Maintenance Blotters, 20/pkg
2308 SPIFE 4000 Gel Staging Lid
2309 SPIFE 4000 Contact Sheets (5/pkg)
2315 SPIFE 4000 White Sample Trays, 10/pkg
2316 SPIFE 4000 Blue Sample Trays, 10/pkg
2319 SPIFE 4000 Applicator Blades
1033 Neutral Density Densitometer Control for SPIFE 4000
9176 Service Manual for SPIFE


Warranty and Service Information


Download Brochure




SPIFE 4000 Specifications

Tests: Serum Proteins and IFE
Media Types: Agarose gels
Maximum Sample Size: 5 x 5 in. (12.7 x 12.7 cm)
Input Power: 120 VAC, 60 Hz, 1000 Watts
230 VAC, 50 Hz, 1000 Watts
Leakage Current: < 3.5 mA. sinusoidal
Dimensions: Height x Width x Depth:
SPIFE 4000 Instrument & Fluids Table:
60 x 79 x 32 in. (152.4 x 200.7 x 81.3 cm)
Computer, Monitor, and Keyboard: 22 x 18 x 24 in. (55.9 x 45.7 x 61 cm)
Scanner: ≤ 8 x 13 x 22 in. (20.3 x 33 x 55.9 cm) Printer: 10 x 14 x 14 in. (25.4 x 35.6 x 35.6 cm)
Total Weight: 350 lbs (158.8 kg)
Environment: 15° to 27°C (59° to 80.6°F)
Instrument Operating Environment:
Ambient Temperature Range:
15° to 27°C (59° to 80.6°F)
Altitude tested up to 2000 meters

Densitometer Specifications
Scanning Parameters:

Gain: Automatic or Manual
Over range: > 100% of full scale
Optics Mode: Visible Transmittance (white light)
Operating System: Microsoft® Windows® 7 *Windows is a registered trademark of Microsoft Inc.
Electrophoresis Timer Range: 1 sec. to 90 min. in 1 second increments
Chamber Temperature Range: 10°C to 30°C Pre-Drying
Temperature Range: 30° to 62°C
Final Drying Temperature Range:
30° to 65°C (86° to 149°F)
Fuses: F1, F2 15A/250V slow blow
Pump System: 2 peristaltic pumps, 1 syringe pump
Test Tubes: 12-16 mm diameter, 75- 100 mm height (Minimum sample volume 500uL)

Electrophoresis Instrument and LIS Interfacing:
3-wire RS232, no hardware handshaking



Auto IFE with SPIFE 4000


SPIFE 4000 automatically performs all the steps for immunofixation.
Auto Dilutions - the sample handler performs all the needed dilutions and applies samples to gels
Auto Antisera - Antisera are automatically applied to gels. Affinity and avidity of the antisera are balanced for best sensitivity with the least amount of blush.
Auto Press and Blot - Assures clear backgrounds, before moving into the auto stainer
Throughput - Four profiles per gel; up to 100 IFEs per 8 hours

Cat. No. Item
2300 40 tests


Auto SPE with SPIFE 4000

No protein retention - application system is unaffected by triglycerides or large proteins.
Active cooling- excellent split-beta separation with no degradation
Great results - resolution and presentation are excellent
Throughput - 28 samples per gel; up to 756 per 8 hours

Cat. No. Item
2310 280 tests


SPIFE 4000 Hemoglobins

SPIFE 4000 Acid Hb - Ten samples per gel with one gel per run.   SPIFE Alkaline Hb - Fourteen samples per gel with up to three gels per run


Alkaline and Acid Hb

SPIFE 4000 can perform both alkaline and acid hemoglobin separations. These assays are automated from sample application to finished gel.

– Aids in identifying hemoglobinopathies
– Stable in storage with acid blue stain
– Excellenct separations

Cat. No. Item
2320 SPIFE 4000 Alkaline HB Kit (80 tests)
2324 SPIFE 4000 Alkaline HB Cassettes with Electrode (1/pkg)
2322 SPIFE 4000 Acid Hb Kit (140 tests)
2315 SPIFE 4000 White Sample Trays (10/pkg)
1359 REP Prepper