Immunofixation
Big News: SPIFE Nexus totally transforms immunofixation into a totally automated assay. What does that mean? You get the full 1-5 mg/dL sensitivity of gel electrophoresis (vs 25 mg/dL with CE) AND reduced labor costs. Just load samples and supplies, press start and return later for a finished gel. Visual status indicators let you see what’s happening at a glance. SPIFE Nexus provides auto sampling, customizable dilution schemes, automated antisera application, staining, destaining and drying. Full-sized gels accomodate up to 15 profiles per gel with smaller sizes available to accommodate any workload.
SPIFE Nexus IFE Kits
Cat. No. / Item / Description
2401 – SPIFE Nexus ImmunoFix-6 (60 Profiles) – 6 samples x 6 channels
2409 – SPIFE Nexus ImmunoFix-9 (90 Profiles) – 9 samples x 6 channels
2408 – SPIFE Nexus ImmunoFix-15 (150 Profiles) – 15 samples x 6 channels
Kits include: 10 Gels, SPIFE Nexus Violet stain, Clear Wash, Citric Acid Destain, Blotters, SPIFE Nexus Pipette Wash, Serrated Applicator Blades, Fixative, and 1 vial each of Antisera to IgG, IgA, IgM, Kappa and Lambda
SPIFE IFE Cup Trays
Cat. No. / Item / Description
3377 – SPIFE IFE-3/6 Dispo Cup Tray
3378 – SPIFE IFE-9 Dispo Cup Tray
3362 – SPIFE IFE-15 Dispo Cup Tray
SPIFE IFE Sample Cups
Cat. No. / Item / Description
3368 – SPIFE IFE-3/6 Dispo Sample Cups
3363 – SPIFE IFE-9/15 Dispo Sample Cups
Additional Supplies and Equipment for SPIFE Nexus IFE Procedures
SPIFE Nexus Antisera Spreader Tips (Cat. No. 2574), SPIFE Nexus Applicator Blade Weights (Cat. No. 2572), SPIFE Nexus Dispo Stain Cups (Cat. No. 2575), REP Prep (Cat. No. 3100), Gel Block Remover (Cat. No.1115), Pos ID Barcode Labels for Touch and SPIFE Nexus Systems 1696
Download Booklet
an immunofixation tutorial
This guide is intended as a primer to the use and interpretation of immunofixation for characterizing monoclonal proteins (M-proteins) in human serum and urine. It contains examples of common and MGUS patterns, as well as those with complex, multiple proteins and light chains. Suggestions for follow-up testing and interpretive comments are included.