Platelet Aggregation

Platelet Aggregation Reagents

Helena's platelet reagents provide outstanding stability and performance at affordable pricing. Reagents are optimized for use with AggRAM, but may be used on any turbidometric aggregometer of similar quality.

Platelet Aggregation Kit

Cat. No. / Item / Description

5369 – Platelet Aggregation Kit

Kit includes: 2 x 1 mL ADP Reagent, 2 x 1 mL Collagen Reagent and 2 x 1 mL Epinephrine Reagent

The Platelet Aggregation Kit is used to identify normal and abnormal platelet function, to quantitate platelet response and to determine the patient's response to anti-platelet drug therapy. The reagents are optimized for use with the Helena AggRAM analyzer, but may be used on any turbidometric aggregometer of similar quality.

ADP Reagent

Cat. No. / Item / Description

5366 – ADP – 2 x 1 mL

ADP is a lyophilized preparation of adenosine-5-diphosphate. ADP is for use in routine platelet aggregation studies for the evaluation of qualitative platelet function disorders. The concentration of the reconstituted ADP reagent is 200 μM.

Collagen Reagent

Cat. No. / Item / Description

5368 – Collagen – 2 x 1 mL

The collagen reagent is a clear, stabilized liquid reagent prepared from equine tendon, which is one of the world's best collagen preparations. Collagen is used in routine platelet aggregation studies for evaluation of qualitative platelet function disorders. The collagen reagent stock solution is reconstituted to a concentration of 100 μg/ml.

Epinephrine Reagent

Cat. No. / Item / Description

5367 – Epinephrine – 2 x 1 mL

Epinephrine is a lyophilized preparation of L-epinephrine bitartrate. Epinephrine is for use in routine platelet aggregation studies for evaluation of qualitative platelet function disorders. After reconstitution, epinephrine concentration is 3 mM.

Arachidonic Acid Reagent

Cat. No. / Item / Description

5364 – Arachidonic Acid – 2 x 1 mL

Arachidonic Acid is a lyophilized preparation of sodium arachidonate for use in platelet aggregation studies of abnormal function, especially dysfunction induced by aspirin and aspirin-containing medications. The concentration of the reconstituted reagent is 5 mg/mL.

Ristocetin Cofactor Reagents and Controls

Ristocetin Cofactor is more sensitive than RIPA to mild reductions in vWF and more often used to monitor therapy in vWD patients. Kit includes ristocetin, formalinized platelets, S.A.R.P., abnormal control and tris-buffered saline.

Helena Ristocetin Cofactor Assay Kit

Cat. No. / Item / Description

5370 – Ristocetin Cofactor Kit, 50 tests

Kit includes: 4 x 5 mL Lyophilized Platelets, 2 x 0.5 mL Ristocetin Cofactor Abnormal Control, 2 x 1 mL S.A.R.P. Assayed Reference Plasma, 2 x 1.5 mL Helena Ristocetin (10 mg/mL), 35 mL Tris-Buffered Saline

Ristocetin cofactor assays measure the ability of von Willebrand factor (Ristocetin cofactor) in the patient's plasma to agglutinate formalinized platelets in the presence of ristocetin. Decreased levels of ristocetin cofactor activity are associated with von Willebrand Disease.

Ristocetin (for Aggregation Assays)

Cat. No. / Item / Description

5199 – Ristocetin – 10 x 0.5 mL

This Ristocetin is for use in platelet aggregation studies for confirmation of von Willebrand Disease (vWD) and Bernard-Soulier Syndrome. Normal platelet-rich plasma exhibits a positive aggregation response to the addition of ristocetin using a final concentration of 12-15 mg/mL. The majority of vWD patients exhibit a negative response to ristocetin as do Bernard-Soulier patients.

Lyophilized Platelets

Cat. No. / Item / Description

5356 – Lyophilized Platelets – 5 x 10 mL
5371 – Lyophilized Platelets – 5 x 5 mL

Lyophilized Platelets are a standardized suspension of fixed platelets derived from platelet concentrate. Lyophilized Platelets are for use in the quantitation of ristocetin cofactor activity in plasma. The platelets are lyophilized for stability. Tris-Buffered Saline (Cat. No. 5365) should be used for reconstitution. The concentration of the reconstituted platelet suspension is approximately 200,000/mL. Platelets in Cat. No. 5356 show a slower reaction rate.

S.A.R.P. Specialty Assayed Reference Plasma

Cat. No. / Item / Description

5185 – S.A.R.P. – 10 x 1 mL

Coagulation S.A.R.P. was the first reference plasma fully assayed for virtually all coagulation studies. S.A.R.P. is prepared from a frozen pool of citrated plasma from healthy donors. S.A.R.P. has normal PT and aPTT clotting times and can be used as reference material for ristocetin and ristocetin cofactor assays.

Ristocetin Cofactor Abnormal Control Plasma

Cat. No. / Item / Description

5373 – Ristocetin Cofactor Abnormal Control – 5 x 0.5 mL

The control is to be used in the assay of ristocetin cofactor. It contains citrated human plasma with a level of ristocetin cofactor activity below 50%.

Ristocetin - 10 mg/mL (for Ristocetin Cofactor Assays)

Cat. No. / Item / Description

5372 – Ristocetin – 10 mg/mL, 5 x 1.5 mL

The reagent is to be used in the quantitative determination of ristocetin cofactor activity. The ristocetin reagent is prepared from Nocardia lurida and is used in the tests for von Willebrand Disease and Bernard-Soulier Syndrome.

Tris-Buffered Saline

Cat. No. / Item / Description

5365 – Tris-Buffered Saline – 1 x 125 mL

Tris-Buffered Saline is used for reconstitution and dilution of some of the reagents used in platelet aggregation assays and ristocetin cofactor assays.