Manual Coagulation Analyzer

Manual Coagulation Analyzer

Cascade M-4 is a manual hemostasis analyzer that is ideal for all routine clotting assays - PTs, aPTTs, thrombins, fibrinogens and factor assays. Designed for easy, inexpensive, low-volume testing, the analyzer is small in size but big on features, offering you many time and labor saving benefits. Operation is simple and economical.

Easy-to-Use Keypad, On-Screen Prompts

All tests are clearly enumerated on the keypad for fast, easy test selection - there's no guess work caused by "hidden" function keys. Computer prompts appear on the screen to conveniently step the user through testing. Default settings are preprogrammed but can easily be changed, if desired.

Procedure Guide

Computerized Features, On-Board QC

Cascade M-4 stores standard curves and calculates patient results, INRs and aPTT ratios. Quality control tasks are easy because Cascade M-4 can calculate mean, SD and CV, plus plot and print Levy-Jennings QC charts. Four Independent photo-optic detection sources read all samples, regardless of turbidity.

Cascade M-4

Compact, Lightweight

With a benchtop footprint smaller than 14" wide by 14" deep and a weight of approximately 21 pounds, Cascade M-4 is sized to fit any lab. Compact size, dependability and versatility make Cascade M-4 an excellent choice as a primary analyzer for smaller laboratories or as a backup unit for larger coagulation analyzers.

Product No. / Item
1711 – Cascade M4, 4-channel analyzer, 6-line screen, 110V / 220V
1718 – Cascade M4 Cuvettes (5 pkgs of 100)
1720 – Cascade M4 Printer Paper (5 rolls)
1475 – Disposable Pipette Tips (50-200 μL), 1000 pkg
9167 – Service Manual for Cascade M4
PT, aPTT, Fibrinogen, Thrombin Clotting Time, Factor Assays
Sample Size:
50 μL, 100 μL, 200 μL
Optics System:
Constant current LED photo optic detection
Temperature Control:
37°C - 0.5°C
Special Features:
Stores standard curves; calculates final results for fibrinogens and factor assays. Calculates INRs and aPTT ratios. On-screen prompts for test instructions. LIS interfacing
Quality Control Features:
Calculates mean, SD, and CV. Plots Levy-Jennings charts
Fluorescent LCD, 6 lines x 40 characters
Accommodates 4 1/2" thermal paper. Reports include date, time, INR, average, technician and patient ID. Prints QC and standard curves
Power Requirements:
110/220 Vac, 50/60 Hz, 100 Watts max
Operating Environment:
Room temperature
8 1/2" H x 14" W x 14" D (22 cm H x 35 cm W x 33 cm D)
21 lbs. (9.5 kg)

Hemostasis Reagents and Controls

Prothrombin Time Assays

Thromboplastin-LI Reagent Kit

Cat. No. / Item / Description

5248 – Thromboplastin-LI Kit, 250 tests – 10 x 5 mL
5249 – Thromboplastin-LI Kit, 500 tests – 10 x 10 mL

Thromboplastin-LI is a stable liquid, ready for use. With a low ISI assignment less than 1.50, this reagent offers reliable sensitivity to liver disease, depression of Vitamin K dependent factors, and coumarin-induced coagulation, even in the presence of high-dose heparin therapy. Open reagent is stable 14 days at 2-8°C.

Activated Partial Thromboplastin Time

aPPT-SA Reagent with Soluble Ellagic Acid Activator

Cat. No. / Item / Description

5388 – aPTT-SA Reagent Kit / 250 tests Ellagic Acid Activated Reagent – 5 x 5 mL / 0.025 M Calcium Chloride – 5 x 5 mL
5389 – aPTT-SA Reagent Kit / 500 tests Ellagic Acid Activated Reagent – 5 x 10 mL 0.025 M Calcium Chloride – 5 x 10 mL
5387 – aPTT-SA Reagent, 1000 tests – 10 x 10 mL
5386 – 0.025 M Calcium Chloride, 1000 tests – 10 x 10 mL

aPTT-SA Reagent uses a soluble ellagic acid activator to provide a more optically clear reagent, an important consideration for automated analyzers with sensitive photo-optical detectors. Ellagic acid reagents also offer excellent precision and sensitivity. aPTT-SA reagent is a stabilized liquid available in three convenient kit sizes.

Fibrinogen and Thrombin Time Assays

Fibrinogen Assay Kit

Cat. No. / Item / Description

5376 – Fibrinogen Assay Kit – 100 tests / Thrombin Reagent – 5 x 2 mL / Fibrinogen Calibrator – 2 x 1 mL / Owren's Veronal Buffer – 2 x 25 mL / Fibrinogen Assay Graph Paper – 1 sheet
5374 – Thrombin Reagent, 200 tests – 10 x 2 mL 0.025 M Calcium Chloride – 5 x 10 mL
5379 –  Fibrinogen Calibrator – 10 x 1 mL

The Fibrinogen Assay kit is for automated, semi-automated and manual quantitation of fibrinogen in plasma. The kit uses a relatively high working concentration of thrombin (100 NIH units/mL) and low fibrinogen concentration to minimize effects of heparin and FDPs. Reagents and calibrator are also available as individual items.

Helena Thrombin Clotting Time (TCT) Reagent

Cat. No. / Item / Description

5377 – Thrombin Time Reagent, 250 tests – 10 x 5 mL

Helena's TCT assay uses a low concentration of thrombin (3 NIH units/mL) with undiluted plasma. It is sensitive to fibrinogen levels below 70 mg/dL. The reagent is ideal for automated, semi-automated and manual instruments.

Factor Deficient Substrates

Congenital Factor Deficient Substrate Plasmas

Cat. No. / Item / Description

5190 – Factor II Deficient Substrate – 10 x 1 mL
5191 – Factor V Deficient Substrate – 10 x 1 mL
5192 – Factor VII Deficient Substrate – 10 x 1 mL
5193 – Factor VIII Deficient Substrate – 10 x 1 mL
5194 – Factor IX Deficient Substrate – 10 x 1 mL
5195 – Factor X Deficient Substrate – 10 x 1 mL
5196 – Factor XI Deficient Substrate – 10 x 1 mL
5197 – Factor XII Deficient Substrate – 10 x 1 mL

For use in Factor Assays, these plasmas are obtained from human congenitally deficient donors (Factor II includes bovine plasma) and have less than 1% residual factor activity.

Owren's Veronal Buffer

Cat. No. / Item / Description

5375 – Owren's Veronal Buffer – 10 x 25 mL

Owren's Veronal Buffer is used as a diluent in preparing patient sample dilutions for Factor Assays and Fibrinogen Assays.

Hemostasis Controls and Reference Plasmas

Hemostasis Controls

Controls for PT, aPTT

Cat. No. / Item / Description

5186 – Norm-Trol 1 – 10 x 1 mL
5187 – Ab-Trol 2 Borderline Control – 10 x 1 mL
5183 – Ab-Trol 3 High Control – 10 x 1 mL

Norm-Trol 1, Ab-Trol 2 and Ab-Trol 3 are normal, moderately elevated, and very elevated controls respectively, for PT and aPTT assays. Norm-Trol 1 is prepared from normal human plasma. Ab-Trol 2 and Ab-Trol 3 are prepared from adsorbed human plasma. Reconstituted controls are stable 8 hours at 2 to 8-C.

S.A.C.-1 and S.A.C.-2 Assayed Controls

Cat. No. / Item / Description

5301 – S.A.C.-1 – 10 x 1 mL
5302 – S.A.C.-2 – 10 x 1 mL

S.A.C.-1 is a normal control plasma assayed for a broad spectrum of hemostasis tests including PT, aPTT, fibrinogen, Proteins C and S, Ristocetin Cofactor, vWF:Ag, plus Factors II, V, VII, VIII, IX, X, XI and XII. S.A.C.-2 is an abnormal control (values < 60% of normal) assayed for the same parameters as S.A.C.-1.

Reference Plasma

S.A.R.P. Specialty Assayed Reference Plasma

Cat. No. / Item / Description

5185 – S.A.R.P. – 10 x 1 mL

Coagulation S.A.R.P. was the first reference plasma fully assayed for virtually all coagulation studies. S.A.R.P. is prepared from a frozen pool of citrated plasma from healthy donors. S.A.R.P. has normal PT and aPTT clotting times and can be used as reference material for the following parameters:

Prothrombin Time Factor V Factor X
Partial Thromboplastin Factor VII FactorXI
Fibrinogen Factor VIII Factor XII  
Factor II Factor IX Ristocetin Cofactor