ELISA Assay Kits

Helena offers premium quality ELISA assay kits for von Willebrand Factor Antigen, Free and Total Protein S Antigen, Protein C Antigen and Monoclonal Free Protein S Antigen. The key advantage of ELISA assays are the fast turnaround time and ease of use. Each kit includes up to 96 tests.


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von Willebrand Factor Antigen (vWF:Ag) by ELISA

Cat. No. / Item / Description

5290 –  vWF:Ag ELISA Kit, 96 tests

Kit includes: 12 x 8-well vWF:Ag Microwell Plate, 60 mL Sample Diluent, 3 x 0.5 mL ELISA Reference Plasma, 12 mL vWF:Ag Conjugate Solution, 13 mL Substrate, 15mL Stopping Solution, 30 mL Phosphate Buffered Saline Concentrate

This vWF:Ag assay uses an ELISA antibody capture sandwich technique to quantitate vWF:Ag in human plasma and is standardized against established reference preparations. Assay time is about an hour and final results are read at 450 nm. vWF:Ag is important in evaluating the pro-coagulant system, and von Willebrand Disease in particular.


Free and Total Protein S Antigen by ELISA

Cat. No. / Item / Description

5292 – Protein S (Free & Total) ELISA Kit, 96 tests

Kit includes: 12 x 8-well Protein S Microwell Plate, 60 mL Sample Diluent, 2 mL Free Protein S Reagent (PEG), 3 x 0.5 mL ELISA Reference Plasma, 12 mL Protein S Conjugate Solution, 13 mL Substrate Solution, 15 mL Stopping Solution, 30 mL Phosphate Buffered Saline

This 90-minute ELISA assay for quantitation of free and total Protein S in human plasma is easy to perform and offers a rapid turnaround time. The assay is read at 450 nm. To determine free Protein S, the physiologically active form, the sample is pretreated with PEG to precipitate the C4b-binding protein complex. Deficiencies of Protein S are associated with increased thrombosis.


Protein C Antigen by ELISA

Cat. No. / Item / Description

5291 –  Protein C ELISA Kit, 96 tests

Kit includes: 12 x 8-well Protein C Microwell Plate, 60 mL Sample Diluent, 3 x 0.5 mL ELISA Reference Plasma, 12 mL Protein C Conjugate Solution, 13 mL Substrate Solution, 15 mL Stopping Solution, 30 mL Phosphate Buffered Saline

This Protein C assay uses an antibody capture sandwich technique to quantitate Protein C in human plasma. Quantitative deficiencies of Protein C are associated with an increased tendency for thrombosis. The Protein C ELISA assay can be completed in about 90 minutes and is read at 450 nm. Its fast turnaround time and ease of use offer advantages over other Protein C assays.


Monoclonal Free Protein S Antigen by ELISA

Cat. No. / Item / Description

5293 – Monoclonal Free Protein S ELISA Kit, 96 tests

Kit includes: 12 x 8-well Free Protein S Microwell Plate, 60 mL Sample Diluent, 3 x 0.5 mL ELISA Reference Plasma, 12 mL Protein S Conjugate Solution, 13 mL Substrate Solution, 15 mL Stopping Solution, 30 mL Phosphate Buffered Saline

This assay uses a monoclonal antibody specific for Free Protein S in an ELISA format to measure Free Protein S directly without PEG precipitation. Incubation times for this procedure total 1 hour, allowing timely completion of testing. Results are read at 450 nm and calculated by comparison to an internationally recognized reference plasma.