Alkaline Phosphatase Isoenzyme
Alkaline phosphatase (ALP) isoenzymes found in human serum originate from several sources with the greatest activity found in bone, liver, intestine, and the placenta. Tissue sources of elevated alkaline phosphatase in serum can be determined by identifying the isoenzyme. The isoenzymes differ in their physicochemical and electrophoretic properties, allowing identification. In addition to liver, bone, intestinal and placental isoenzymes, macrohepatic, Regan, PA, Nagao, and renal isoenzymes have been identified. These isoenzymes can be identified using the SPIFE Nexus analyzer.
SPIFE Nexus ALP agarose gels have a unique additive in the gel matrix to ensure separation of macrohepatic from liver and bone fractions. Sample pre-treatment with neuraminidase provides greater separation. SPIFE Nexus automates all steps of testing.
SPIFE Nexus Alkaline Phosphatase Isoenzyme Kits
Cat. No. / Item / Description
2345 – SPIFE Nexus ALP-20 Kit – 10 x 20 samples/gel
2346 – SPIFE Nexus ALP-40 Kit – 10 x 40 samples/gel
Kits include: 10 Gels, ALP Reagent, ALP Diluent, Blotters, Citric Acid Destain, Applicator Blades, Plastic reagent vials.
Alkaline Phosphatase Isoenzyme Control
Cat. No. / Item / Description
5104 – Gel ALP Isoenzyme Control– 1 x 2 mL
This control is for use as a qualitative and/or quantitative control to aid in the identification of alkaline phosphatase isoenzymes by agarose gel electrophoresis. It is prepared from pooled human serum and contains liver and bone isoenzyme bands. The control is a stabilized liquid.
SPIFE ALP Separation Enhancer
Cat. No. / Item / Description
3348 – SPIFE ALP Separation Enhancer – 1 x 4 mL
The neuraminidase in the Separation Enhancer causes a greater reduction in mobility of the bone enzyme than the liver isoenzyme upon electrophoresis. 50 μL of patient sample is pre-treated with 10 μL of separation enhancer within 10 minutes of electrophoresis, to provide greater separation of bone and liver isoenzymes.
Additional Supplies and Equipment for SPIFE Nexus ALP Procedures:
REP Prep (Cat. No. 3100), SPIFE Nexus Applicator Blade Weights (Cat. No. 2572), SPIFE 20,40,60 Dispo Cup Tray (Cat. No. 3370), SPIFE Dispo Cups (Deep Well - Cat. No. 3360), SPIFE Nexus Applicator Templates (Cat. No. 2570), SPIFE Nexus Dispo Stain Cups (Cat. No. 2575), Pos ID Barcode Labels for Touch and SPIFE Nexus Systems (Cat. No. 1696) SPIFE Nexus Reagent Roller (Cat. No. 2583), SPIFE Nexus Carbon Electrode Insert (Cat. No. 2576)