V8 capillary


SPIFE 3000 Assays

V8 brochure


SPIFE 3000 Workflow

The SPIFE 3000 separates and develops up to 1400 SPE samples or 210 IFE profiles in an 8-hour shift, with integrated sample and reagent application. Its barcode "lockout" feature provides positive ID between sample tray and electrophoresis gel. Click to see full workflow diagram.

ife profiles




Electrophoresis Auto Sample Handler

The Electrophoresis Auto Sample Handler provides automated front-end specimen processing for SPIFE 2000/3000, easily accommodating all standard blood collection tubes. The Auto Sample Handler is equipped with a liquid level sensor, and provides positive patient ID with a barcode reader with LIS interface. The Auto Sample Handler transfers specimens directly from primary tubes to the applicator base or tray in efficient preparation for SPIFE 3000. The Auto Sample Handler can also automatically prepare dilutions for IFEs.


sampler handler


- Primary tube sampling with liquid level sensor
- Barcode reader with LIS interface for positive ID
- Automatically performs dilutions for IFEs
- Samples loaded to barcoded sample tray ready for application
- Interfaces to QuickScan 2000 or LIS for workload listing

Electrophoresis Auto Sampler Specifications
Test Tubes: 13-16 mm diameter; 75-100 mm height
Minimum Sample Volume : 500 μL
Operating Environment: 59- to 81- F (15- to 27-C)
Dimensions: 19" H x 30" W x 26" D (48 x 76 x 66 cm)
Computer System: Integral AT-compatible with 3-" disk drive
Interface: 3-wire RS232, bidirectional, LIS or QuickScan 2000
Bar Codes : Code 3 of 6 (Code 9) (alphanumeric), Codabar (numeric), Interleaved 2 of 5 (numeric), L reduced (numeric), Code 128 (alphanumeric)
Input Power: 120/240 (85-264) Vac, 50/60 Hz
Display: 2x40-character LCD
Weight: 100 lbs. (45.4 kg)

Cat. No. Item
1341 Electrophoresis Auto Sampler Handler, 110/22V
1665 Omterface Cable fpr EASH/QS 2000
1666 Pos ID Barcode Labels, 1000/pkg
1669 Pos ID Barcode Readers & Cabling, 3 sets




Helena SPIFE 3000

Separations are superior and we continually look for ways to increase efficiency and labor savings. New urine protein and immunofixation assays that require no sample concentration are just one example.

Designed with You in Mind
Besides being a superior system for Cholesterols, IFE, Proteins and Hemoglobins, new menu choices such as IgG IEF, CK, LD, Alk Phos Isoenzymes and Lipoproteins add to the versatility of the system. New gel sizes - from 10 sample to 100 sample - meet any testing volume.

What's New

- Urine Protein and IFE using unconcentrated urine
- High Resolution Proteins with better resolution and separation for easier
differentiation of glomerular, tubular and mixed proteinuria
- Pentavalent IFE antisera available for monoclonal protein screening
- QuickGel SPE, IFE and Hemoglobin assays are ideal for low volumes or quick turnaround.
- 10-profile IgG IEF gels use unconcentrated CSF for diagnosing multiple sclerosis

Easy, Accurate Sampling -
The integrated applicator precisely applies up to 100 samples at once - fast, clean and accurate. Mylar tips prevent protein adherence that can cause erroneous results.

Auto Reagent Application -
Reagent is automatically applied to gels for enzymatic assays.

Great Results-
Gels are processed automatically with excellent final results & a low cost per test.

Positive ID -
SPIFE 3000 compares the barcodes from the sample tray and gel - if no match, “lockout” prevents errors.-

On-line Availability -
SPIFE provides automated access to two on-line stains - neat and efficient.



    Accurate Sampling

    The integrated applicator precisely applies up to 100 samples at once - fast, clean and accurate.


    Positive ID

    SPIFE 3000 compares the barcodes from the sample tray and gel - if no match, "lockout" prevents errors.


    Reagent and Stain

    Reagent is automatically applied to gels for enzymatic assays. On-line stains for visible analytes are also available.

SPIFE 3000 Specifications

Test Types: Proteins, Immunofixation, High Resolution Proteins, Acid Hemoglobins, Alkaline Hemoglobins, Cholesterol Profile (HDL, VLDL, LDL, Lp(a), CK Isoenzymes, LD Isoenzymes, Alkaline Phosphatase Isoenzymes, Lipoproteins, IgG Isoelectric Focusing
Displays: Two, 2 x 40 character LCD
Timer Range: 00:00 to 99:59 min:sec in 1 second increments
Chamber Temperature Range: 50- to 144-F (10- to 62-C)
Dryer Temperature Range: 86- to 158-F (30- to 70-C)
Input Power: 120 Vac (Cat. No. 1088) or 240 Vac (Cat. No. 1089); 50/60 Hz
Voltage Tolerance: 3%
Power Range: 2000 VA
Isolation: 4000 VRMS
Leakage Current: -Less than 50:A
Operating Environment:- 59- to 80-F (15- to 27-C)
Dimensions: 12" H x 36" W x 21" D (30 x 91 x 53 cm)
Weight: 78 lbs. (35.4 kg)

Cat. No. Item
-1088 -SPIFE 3000, 110V
-1089 -SPIFE 3000, 220V


SPIFE Accessories

Cat. No. Item
1093 SPIFE Replacement Pump
1113 SPIFE Maintenance Swabs (5/pkg)
1115 SPIFE Gel Block Remover
1119 SPIFE Antisera Tray for IFE-6/3
1122 SPIFE IFE Multi-Channel Pipette
1361 REP/SPIFE Contact Sheets (5/pkg)
1346 Barcode Reader & Cabling (1 set)
1359 REP Prepper
1666 Pos ID Barcode Labels (pkg of 1000)
1669 Pos ID Barcode Readers & Cabling (3 sets)
3349 Tip Spacers for SPIFE IFE-6/3 Multi-Channel Pipette (3/pkg)
3352 SPIFE Rigid Antisera Template-15
3355 Tips for IFE-15 Multi-Channel Pipette
3356 Tip Spacers for SPIFE IFE-9/15 Multi-Channel Pipette (3/pkg)
3388 SPIFE Disposable Stainless Steel Electrodes (3/pkg)
3394 SPIFE IFE-9 Antisera Tray
3395 SPIFE IFE-3 Rigid Antisera Template
3396 Tip Spacers for SPIFE IFE-9 Multi-Channel Pipette
3397 Tips for IFE-9 Multi-Channel Pipette (rack of 384)
3402 Tips for IFE-6/3 Multi-Channel Pipette (10 pks of 72)
3410 SPIFE IFE-6 Rigid Antisera Template
3383 SPIFE IgG IEF Electrodes, 2/pkg
3386 SPIFE Reagent Spreader (for SPIFE 2000)
3387 Applicator Blade Weights (for SPIFE, REP) (3/pkg)
3392 SPIFE IFE-9 Rigid Antisera Template
3394 SPIFE IFE-9 and 15 Antisera Tray
3450 SPIFE Applicator Blades, 30/pkg
3451 SPIFE Modified Applicator Blades, 30/pkg
3407 SPIFE Urine IFE-15 Alignment Guide
3703 SPIFE IgG IEF Square Replacement Electrode
3704 SPIFE IEF Electrodes/Adapters (3 electrodes/2 adapters)
3706 Reagent Spreaders (2/pkg)
3709 SPIFE Replacement Electrodes (2/pkg)
6300 10-100 μL Variable Volume Pipette with Tip Ejector

Warranty and Service Information


Dispo Cups and Cup Trays for SPIFE, SPIFE 2000, SPIFE 3000

Cat. No. Item
1343 SPIFE/REP 20, 40, 60, Dispo Cup Tray-
3353 SPIFE QuickGel Dispo Cup Tray, Row 6/12-
3354 SPIFE QuickGel Dispo Cup Tray, Row 8/B-
3361 SPIFE 20 Dispo Cup Tray-
3362 SPIFE IFE-15 Dispo Cup Tray-
3364 SPIFE 20-80 Dispo Cup Tray
3366 SPIFE 20-100 Dispo Cup Tray-
3367 SPIFE IFE-9 Dispo Cup Tray-
3370 SPIFE 20, 40, 60, Dispo Cup Tray-
3376 SPIFE IFE-3/6 Dispo Cup Tray-
3377 SPIFE IFE-3/6 Dispo Cup Tray
3378 SPIFE IFE-9 Dispo Cup Tray
3360 SPIFE Dispo Sample Cups - Deep Well, 100/pkg
3363 SPIFE IFE - 9/15 Dispo Sample Cups, 100/pkg
3368 SPIFE IFE - 3/6 Dispo Sample Cups, 100/pkg
3369 SPIFE Dispo Sample Cups - Shallow Well, 100/pkg


SPIFE Accessories for QuickGel

Cat. No. Item
1111 SPIFE QuickGel Electrodes, 2/pkg
3353 SPIFE QuickGel Dispo Cup Tray, Row 6/12
3354 SPIFE QuickGel Dispo Cup Tray, Row 8/B
3357 SPIFE QuickGel Dispo Stainless Steel Electrodes, 3/pkg
3358 SPIFE QuickGel Holder, 1/pkg
3371 SPIFE QuickGel IFE Sample Tray
3372 SPIFE QuickGel Rigid Antisera Template
3373 SPIFE QuickGel IFE Alignment Guide