ELISA Assay Kits

Helena offers premium quality ELISA assay kits for von Willebrand Factor Antigen, Free and Total Protein S Antigen, Protein C Antigen and Monoclonal Free Protein S Antigen. The key advantage of ELISA assays are the fast turnaround time and ease of use. Each kit includes up to 96 tests.


von Willebrand Factor Antigen by ELISA


von Willebrand Factor Antigen (vWF:Ag) by ELISA

Cat. No. / Item / Description

5290 –  vWF:Ag ELISA Kit, 96 tests

Kit includes: 12 x 8-well vWF:Ag Microwell Plate, 60 mL Sample Diluent, 3 x 0.5 mL ELISA Reference Plasma, 12 mL vWF:Ag Conjugate Solution, 13 mL Substrate, 15mL Stopping Solution, 30 mL Phosphate Buffered Saline Concentrate

This vWF:Ag assay uses an ELISA antibody capture sandwich technique to quantitate vWF:Ag in human plasma and is standardized against established reference preparations. Assay time is about an hour and final results are read at 450 nm. vWF:Ag is important in evaluating the pro-coagulant system, and von Willebrand Disease in particular.


Free and Total Protein S Antigen by ELISA

Cat. No. / Item / Description

5292 – Protein S (Free & Total) ELISA Kit, 96 tests

Kit includes: 12 x 8-well Protein S Microwell Plate, 60 mL Sample Diluent, 2 mL Free Protein S Reagent (PEG), 3 x 0.5 mL ELISA Reference Plasma, 12 mL Protein S Conjugate Solution, 13 mL Substrate Solution, 15 mL Stopping Solution, 30 mL Phosphate Buffered Saline

This 90-minute ELISA assay for quantitation of free and total Protein S in human plasma is easy to perform and offers a rapid turnaround time. The assay is read at 450 nm. To determine free Protein S, the physiologically active form, the sample is pretreated with PEG to precipitate the C4b-binding protein complex. Deficiencies of Protein S are associated with increased thrombosis.


Protein C Antigen by ELISA

Cat. No. / Item / Description

5291 –  Protein C ELISA Kit, 96 tests

Kit includes: 12 x 8-well Protein C Microwell Plate, 60 mL Sample Diluent, 3 x 0.5 mL ELISA Reference Plasma, 12 mL Protein C Conjugate Solution, 13 mL Substrate Solution, 15 mL Stopping Solution, 30 mL Phosphate Buffered Saline

This Protein C assay uses an antibody capture sandwich technique to quantitate Protein C in human plasma. Quantitative deficiencies of Protein C are associated with an increased tendency for thrombosis. The Protein C ELISA assay can be completed in about 90 minutes and is read at 450 nm. Its fast turnaround time and ease of use offer advantages over other Protein C assays.


Monoclonal Free Protein S Antigen by ELISA

Cat. No. / Item / Description

5293 – Monoclonal Free Protein S ELISA Kit, 96 tests

Kit includes: 12 x 8-well Free Protein S Microwell Plate, 60 mL Sample Diluent, 3 x 0.5 mL ELISA Reference Plasma, 12 mL Protein S Conjugate Solution, 13 mL Substrate Solution, 15 mL Stopping Solution, 30 mL Phosphate Buffered Saline

This assay uses a monoclonal antibody specific for Free Protein S in an ELISA format to measure Free Protein S directly without PEG precipitation. Incubation times for this procedure total 1 hour, allowing timely completion of testing. Results are read at 450 nm and calculated by comparison to an internationally recognized reference plasma.