Alkaline phosphatase (ALP) in human serum originates from several tissue sources with the greatest activity resulting from bone, liver, intestine and placenta. Because of the wide distribution, limited information can be obtained from a total ALP assay. Fortunately the tissue sources of elevated ALP in serum can be determined by identifying the isoenzyme. ALP isoenzymes differ in their physicochemical and electrophoretic properties, and by taking advantage of these differences, individual isoenzymes can be identified. In addition to liver, bone, intestinal and placental isoenzymes, Regan, PA, Nagao and renal isoenzymes have also been identified.
Titan III Cellulose Acetate ALP Procedure Total Time: approximately 50 minutes.
See also: SPIFE Alkaline Phosphatase Assay
|5102||ALP Indolyl Blue Reagent|
|-||10 x 3 mL Reagent|
|-||1 x 30 mL Diluent|
This reagent is for colorimetric development of ALP isoenzyme bands after electrophoresis. Results obtained with Indolyl blue are easily visualized and interpreted due to the uniform white background and bright intense bands. It can be used instead of the traditional reagents listed below.
|5139||ALP Isoenzyme Control, 1 x 1 mL|
The control contains liver and intestinal alkaline phosphatase isoenzymes. It is prepared from tissue homogenates and is for qualitative and quantitative use with the Titan III cellulose acetate system. The control is a stabilized liquid.