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V8 capillary

 

SPIFE 3000 Assays

 

SPIFE 3000


The SPIFE 3000 separates and develops up to 1400 SPE samples or 210 IFE profiles in an 8-hour shift, with integrated sample and reagent application. Its barcode "lockout" feature provides positive ID between sample tray and electrophoresis gel.

 


An Immunofixation Tutorial

This guide is intended as a primer to the use and interpretation of immunofixation for characterizing monoclonal proteins (M-proteins) in human serum and urine. It contains examples of common and MGUS patterns, as well as those with complex, multiple proteins and light chains. Suggestions for follow-up testing and interpretive comments are included.

immunofix tutorial

SPIFE ImmunoFix

Spife ImmunoFix Gel

 

 

 

 

 

 

 

 

 

 

• 3, 6, 9 and 15-profile gels accommodate a wide range of test volumes
• New 2-profile QuickGel IFE available
• Profile IgG, IgA, IgM, k and l on up to 15 samples at one time for maximum throughput of up to 210 profiles in a single 8-hour shift
• Multichannel pipetting simplifies application of color-coded anitsera
• Affinity & avidity of antisera balanced for highest sensitivity with least amount of background blush
• Resolution & sensitivity of gel provide tight crisp bands
• Acid violet stain enhances visualization

 

 

 


SPIFE ImmunoFix Kits

Cat. No. Item Description
3408 SPIFE ImmunoFix-15 (150 Profiles) 15 samples x 6 channels
3409 SPIFE ImmunoFix- 9 (90 Profiles) 9 samples x 6 channels
3401 SPIFE ImmunoFix- 6 (60 Profiles) 6 samples x 6 channels
3406 SPIFE ImmunoFix- 3 (30 Profiles) 3 samples x 6 channels

 

SPIFE ImmunoFix Kits include: 10 Gels, Acid Violet Stain, Blotters, Tris-Buffered Saline, Citric Acid Destain, Applicator Blades, Blotter Combs, Applicator Swabs, Fixative, and 1 vial each of Antisera to IgG, IgA, IgM, Kappa and Lambda.
NOTE:
SPIFE ImmunoFix Gels and Antisera may be purchased separately:

Cat. No. Item
3413 SPIFE IFE Antisera Kit (3 mL ea Fixative, IgG, IgA, IgM, K, λ)
3465 SPIFE IFE Light Chain Antisera Kit (3 mL ea Fixative, K, λ)
3466 SPIFE IFE-3 Gels (10 gels w/ consumables, no antisera)
3411 SPIFE IFE-6 Gels (10 gels w/ consumables, no antisera)
3469 SPIFE IFE-9 Gels (10 gels w/ consumables, no antisera)

 

 


 

QuickGel IFE Kit for SPIFE

QuickGel IFE Kit for Spife

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Cat. No. Item
3351 QuickGel IFE Kit - SPIFE

 

Kits include: 10 QuickGel IFE Gels, Acid Violet Stain, Tris-Buffered Saline, Citric Acid Destain, QuickGel Blotters, QuickGel IFE Blotter Combs, 20 Applicator Blades and 1 vial each Fixative, IgG, IgA, IgM, Kappa and Lambda antisera.
Additional Supplies and Equipment for QuickGel IFE SPIFE Procedure:
REP Prep (Cat. No. 3100), SPIFE QuickGel IFE Cup Tray (Cat. No. 3371), SPIFE IFE 9/15 Dispo Cups (Cat. No. 3363), QuickGel Gel Block Remover (Cat. No. 1262), SPIFE QuickGel IFE Antisera Template (Cat. No. 3372), SPIFE QuickGel Electrodes (Cat. No. 1111), SPIFE QuickGel Gel Holder (Cat. No. 3358), SPIFE QuickGel IFE Chamber Alignment Guide (Cat. No. 3373).

 

 


Supplies - SPIFE ImmunoFix - 15/9/6/3

Additional Supplies and Equipment for SPIFE ImmunoFix-15

Cat. No. Item
1122 SPIFE IFE Multi-Channel Pipette
3356 IFE 9/15 Tip Spacers for SPIFE Multi-Channel Pipette (3/pkg)
3355 Tips for SPIFE Multi-Channel Pipette (4 x 204)
3407 SPIFE Urine IFE-15 Alignment Guide
3352 SPIFE Rigid Antisera Template-15
3362 SPIFE 3000 IFE-15 Dispo Cup Tray
3363 SPIFE IFE-9/15 Dispo Cups
3394 SPIFE IFE-9 Antisera Tray

 

Additional Supplies and Equipment for SPIFE ImmunoFix-9

Cat. No. Item
1122 SPIFE IFE Multi-Channel Pipette
3356 IFE 9/15 Tip Spacers for SPIFE Multi-Channel Pipette (3/pkg)
3355 Tips for SPIFE Multi-Channel Pipette (4 x 204)
3392 IFE-9 Antisera Template
3394 IFE-9 Antisera Tray
3378 SPIFE IFE-9 Dispo Cup Tray
3363 SPIFE IFE-9/15 Dispo Cups 
3391 SPIFE Urine IFE-9 Accessories (50 templates and blotters)

 

Additional Supplies and Equipment for SPIFE ImmunoFix-6 or -3

Cat. No. Item
1122 SPIFE IFE Multi-Channel Pipette
3349 IFE 6/3 Tip Spacers for SPIFE Multi-Channel Pipette (3/pkg)
3355 Tips for SPIFE Multi-Channel Pipette (4 x 204)
3410 IFE-6 Antisera Template
3395 IFE-3 Antisera Template
1119 IFE Antisera Tray
3390 SPIFE Urine IFE-3/6 Accessories (50 templates and blotters)

 

Additional Supplies and Equipment for SPIFE ImmunoFix Procedures: REP Prep (Cat. No. 3100), Gel Block Remover (Cat. No. 1115).

Cat. No. Item
3396 IFE-9 Tip Spacers for SPIFE IFE-9 Pipette - Old Style (3/pkg)
3397 IFE-9 Tips for SPIFE IFE-9 Pipette - Old Style (rack of 384)
3402 Tips for IFE 6/3 Multi-Channel Pipette - Old Style (10 pkgs of 72)
3377 SPIFE 2000/3000 IFE 3/6 Dispo Cup Tray
3368 SPIFE IFE 3/6 Dispo Cups

 

 


 

Additional Antisera for use with SPIFE ImmunoFix

Cat. No. Item Description
9409 IFE Antiserum to IgD 1 x 1 mL
9410 IFE Antiserum to IgE 1 x 1 mL
9412 IFE Free Kappa Antiserum 1 x 1 mL
9413 IFE Free Lambda Antiserum 1 x 1 mL

 

  • Antisera for Spife ImmunoFix

When performing IFE, if a reaction occurs with Kappa or Lambda but there's no reaction with IgG, IgA or IgM heavy chains, the sample should be tested with IgD and IgE antisera. A reaction indicates an IgD or IgE monoclonal gammopathy. A light chain reaction with no IgD or IgE reaction indicates Free Light Chain Disease.

For use with serum or urine, the Free Kappa and Lambda Antisera are specific for free kappa and free lambda light chains respectively, reacting only with free light chains not attached to the heavy chain portion of the molecule.

 

ImmunoFix Quality Control

Cat. No. Item Description
9400 ImmunoFix Control Kit 1 x 0.5 mL IgG Kappa Control
    1 x 0.5 mL IgA Lambda Control
    1 x 0.5 mL IgM Control

 

  • ImmunofFix Quality Control

ImmunoFix Controls are used to confirm antisera reactivity. After electrophoresis, the appropriate control is applied to a well at the end of each channel on the gel. Precipitin rings form around wells, indicating proper antigen/antibody reactions. Controls can also be treated as a patient sample to verify all phases of IFE procedure.