SPIFE ImmunoFix GelsSPIFE ImmunoFix

3, 6, 9 and 15-profile gels accommodate a wide range of test volumes
New 2-profile QuickGel IFE available
Profile IgG, IgA, IgM, k and l on up to 15 samples at one time for
maximum throughput of up to 210 profiles in a single 8-hour shift
Multichannel pipetting simplifies application of color-coded anitsera
Affinity & avidity of antisera balanced for highest sensitivity with least
  amount of background blush
Resolution & sensitivity of gel provide tight crisp bands
Acid violet stain enhances visualization

 


SPIFE ImmunoFix Kits

Cat. No. Item Description
3408 SPIFE ImmunoFix-15 (150 Profiles) 15 samples x 6 channels
3409 SPIFE ImmunoFix- 9 (90 Profiles) 9 samples x 6 channels
3401 SPIFE ImmunoFix- 6 (60 Profiles) 6 samples x 6 channels
3406 SPIFE ImmunoFix- 3 (30 Profiles) 3 samples x 6 channels

SPIFE ImmunoFix Kits include: 10 Gels, Acid Violet Stain, Blotters, Tris-Buffered Saline, Citric Acid Destain, Applicator Blades, Blotter Combs, Applicator Swabs, Fixative, and 1 vial each of Antisera to IgG, IgA, IgM, Kappa and Lambda.
NOTE:
SPIFE ImmunoFix Gels and Antisera may be purchased separately:

Cat. No. Item  
3413 SPIFE IFE Antisera Kit (3 mL ea Fixative, IgG, IgA, IgM, K, λ)  
3465 SPIFE IFE Light Chain Antisera Kit (3 mL ea Fixative, K, λ)  
3466 SPIFE IFE-3 Gels (10 gels w/ consumables, no antisera)  
3411 SPIFE IFE-6 Gels (10 gels w/ consumables, no antisera)  
3469 SPIFE IFE-9 Gels (10 gels w/ consumables, no antisera)  

 

QuickGel IFE Kit for SPIFEQuickGel IFE Kit for SPIFE

Cat. No. Item
3351 QuickGel IFE Kit - SPIFE

Kits include: 10 QuickGel IFE Gels, Acid Violet Stain, Tris-Buffered Saline, Citric Acid Destain, QuickGel Blotters, QuickGel IFE Blotter Combs, 20 Applicator Blades and 1 vial each Fixative, IgG, IgA, IgM, Kappa and Lambda antisera.
Additional Supplies and Equipment for QuickGel IFE SPIFE Procedure:
REP Prep (Cat. No. 3100), SPIFE QuickGel IFE Cup Tray (Cat. No. 3371), SPIFE IFE 9/15 Dispo Cups (Cat. No. 3363), QuickGel Gel Block Remover (Cat. No. 1262), SPIFE QuickGel IFE Antisera Template (Cat. No. 3372), SPIFE QuickGel Electrodes (Cat. No. 1111), SPIFE QuickGel Gel Holder (Cat. No. 3358), SPIFE QuickGel IFE Chamber Alignment Guide (Cat. No. 3373).

 

Additional Supplies and Equipment for SPIFE ImmunoFix-15

Cat. No. Item  
1122 SPIFE IFE Multi-Channel Pipette  
3356 IFE 9/15 Tip Spacers for SPIFE Multi-Channel Pipette (3/pkg)  
3355 Tips for SPIFE Multi-Channel Pipette (4 x 204)  
3407 SPIFE Urine IFE-15 Alignment Guide  
3352 SPIFE Rigid Antisera Template-15  
3362 SPIFE 3000 IFE-15 Dispo Cup Tray  
3363 SPIFE IFE-9/15 Dispo Cups  
3394 SPIFE IFE-9 Antisera Tray  

Additional Supplies and Equipment for SPIFE ImmunoFix-9

Cat. No. Item  
1122 SPIFE IFE Multi-Channel Pipette  
3356 IFE 9/15 Tip Spacers for SPIFE Multi-Channel Pipette (3/pkg)  
3355 Tips for SPIFE Multi-Channel Pipette (4 x 204)  
3392 IFE-9 Antisera Template  
3394 IFE-9 Antisera Tray  
3378 SPIFE IFE-9 Dispo Cup Tray  
3363 SPIFE IFE-9/15 Dispo Cups   
3391 SPIFE Urine IFE-9 Accessories (50 templates and blotters)  

Additional Supplies and Equipment for SPIFE ImmunoFix-6 or -3

Cat. No. Item  
1122 SPIFE IFE Multi-Channel Pipette  
3349 IFE 6/3 Tip Spacers for SPIFE Multi-Channel Pipette (3/pkg)  
3355 Tips for SPIFE Multi-Channel Pipette (4 x 204)  
3410 IFE-6 Antisera Template  
3395 IFE-3 Antisera Template  
1119 IFE Antisera Tray  
3390 SPIFE Urine IFE-3/6 Accessories (50 templates and blotters)  

Additional Supplies and Equipment for SPIFE ImmunoFix Procedures: REP Prep (Cat. No. 3100), Gel Block Remover (Cat. No. 1115).

Cat. No. Item  
3396 IFE-9 Tip Spacers for SPIFE IFE-9 Pipette - Old Style (3/pkg)  
3397 IFE-9 Tips for SPIFE IFE-9 Pipette - Old Style (rack of 384)  
3402 Tips for IFE 6/3 Multi-Channel Pipette - Old Style (10 pkgs of 72)  
3377 SPIFE 2000/3000 IFE 3/6 Dispo Cup Tray  
3368 SPIFE IFE 3/6 Dispo Cups  

Additional Antisera for use with SPIFE ImmunoFix

  • IFE Antiserum to IgD and IgE, IFE Free Kappa and Lamda AntiserumAdditional Antisera for use with SPIFE ImmunoFix

    Cat. No. Item Description
    9409 IFE Antiserum to IgD 1 x 1 mL
    9410 IFE Antiserum to IgE 1 x 1 mL
    9412 IFE Free Kappa Antiserum 1 x 1 mL
    9413 IFE Free Lambda Antiserum 1 x 1 mL

    When performing IFE, if a reaction occurs with Kappa or Lambda
    but there's no reaction with IgG, IgA or IgM heavy chains, the sample should be tested with IgD and IgE antisera. A reaction indicates an IgD or IgE monoclonal gammopathy. A light chain reaction with no IgD or IgE reaction indicates Free Light Chain Disease.

    For use with serum or urine, the Free Kappa and Lambda Antisera are specific for free kappa and free lambda light chains respectively, reacting only with free light chains not attached to the heavy chain portion of the molecule.

ImmunoFix Quality Control

ImmunoFix Control KitImmunoFix Quality Control

Cat. No. Item Description
9400 ImmunoFix Control Kit 1 x 0.5 mL IgG Kappa Control
    1 x 0.5 mL IgA Lambda Control
    1 x 0.5 mL IgM Control



ImmunoFix Controls are used to confirm antisera reactivity. After electrophoresis, the appropriate control is applied to a well at the end of each channel on the gel. Precipitin rings form around wells, indicating proper antigen/antibody reactions. Controls can also be treated as a patient sample to verify all phases of IFE procedure.

SPIFE 3000

The SPIFE 3000 separates and develops up to 1400 SPE samples or 210 IFE profiles in an 8-hour shift, with integrated sample and reagent application. Its barcode "lockout" feature provides positive ID between sample tray and electrophoresis gel.

An Immunofixation Tutorial

This guide is intended as a primer to the use and interpretation of immunofixation for characterizing monoclonal proteins (M-proteins) in human serum and urine. It contains examples of common and MGUS patterns, as well as those with complex, multiple proteins and light chains. Suggestions for follow-up testing and interpretive comments are included.

Intergrated Brochure

An Immunofiation Tutorial