Hemoglobins
Helena can meet all your needs for identifying hemoglobin variants, taking full advantage of the automated sample application separation and staining of the SPIFE electrophoresis system. Both alkaline and acid methods are available.
Alkaline Hemoglobin
Separations with SPIFE Alkaline Hemoglobin mimic classic cellulose acetate and are the front line for detecting hemoglobinopathies. Use of acid blue stain provides excellent sensitivity and linearity. Precise temperature control improves separation, resolving even problematic Hemoglobin H.
SPIFE Alkaline Hemoglobin Kits
Cat. No. / Item / Description
3415 – SPIFE Alk Hb - 20 Kit – 10 x 20 samples
3429 – QuickGel Alkaline Hemoglobin Kit for SPIFE – 10 samples
Kits include: 10 Gels, Acid Blue Stain, REP/SPIFE Hemolysate, Citric Acid Destain, Applicator Blades.
For QuickGel Alkaline Hemoglobin Kit for QuickGel Chamber, see Cat. No. 3529 and 3529T.
Acid Hemoglobin
SPIFE Acid Hemoglobin is a much less cumbersome method than conventional citrate agar for characterizing hemoglobinopathies. The use of acid blue stain brings linearity not available with O-Tolidine or O-Dianisidine based systems. Finished gels are stable in storage and will not continue to darken.
SPIFE Acid Hemoglobin Kit
Cat. No. / Item / Description
3418 – SPIFE Acid Hemoglobin Kit – 10 x 20 samples
3419 – QuickGel Acid Hemoglobin Kit for SPIFE – 10 x 10/20 samples*
Kits include: 10 Gels, Acid Blue Stain, REP/SPIFE Hemolysate, Citric Acid, Destain, Applicator Blades.
*Additional SPIFE Applicator Blades (Cat. No. 3450) can be purchased to accommodate up to 20 samples per gel.
For QuickGel Acid Hemoglobin Kit for QuickGel Chamber, see Cat. No. 3519.
Additional Supplies and Equipment for SPIFE Hemoglobin Procedures:
AFSC Hemo Control (Cat. No. 5331), AA2 Hemo Control (Cat. No. 5328), REP Prep (Cat. No. 3100), Gel Block Remover (Cat. No. 1115), Applicator Blade Weights (Cat. No. 3387), Disposable Sample Cups (Cat. No. 3369), SPIFE Disposable Cup Tray (Cat. No. 3370)
Additional Supplies and Equipment for QuickGel Hemoglobin Procedures:
AFSC Hemo Control (Cat. No. 5331), AA 2 Hemo Control (Cat. No. 5328), REP Prep (Cat. No. 3100), SPIFE QuickGel Electrode (Cat. No. 1111), SPIFE QuickGel Holder (Cat. No. 3358), QuickGel Gel Block Remover (Cat. No. 1262),SPIFE QuickGel Chamber Alignment Guide (Part No. 86541003), Applicator Blade Weights (Cat. No. 3387), Disposable Sample Cups (Cat. No. 3369), QuickGel Dispo Cup Tray (Cat. No. 3353), Chamber Cover Part No. 8JP34012)
Download Hemoglobin Wallchart
relative hemoglobin mobilities by gel electrophoresis
This chart provides a handy reference to the relative electrophoretic mobility of hemoglobins in both an acid and alkaline system. Fifty of the more common hemoglobin variants are represented.