Helena SPIFE 4000

Total Automation for IFEs and SPEs
Yes, you really can just load the sample tubes, press go, and return for your IFE and SPE final results! SPIFE 4000 sets the new standard in automated electrophoresis by eliminating ALL manual steps – there’s no manual sample application, no stains or antisera to add, no gel transfers, no dilutions to perform, no gel blocks to remove, no tech-to-tech variability – just load and walk away. In a single 8-hour shift, SPIFE 4000 can turn 100 IFEs or 756 SPEs. With SPIFE 4000’s hands-off approach, a laboratory could reasonably expect to be able to run 24 hours per day 7 days a week.

A full run takes about 30 minutes of tech time for set up, then frees the tech to perform other tasks with NO disruptions to transfer gels or scan. All materials are loaded pre-run for uninterrupted operation – no coming back to apply antisera, blotters or reagents. Not only does SPIFE 4000 provide fully automated electrophoresis with good throughput at a reasonable price, the results are excellent. There’s no protein retention in the application system, the resolution is superb, and antisera affinity and avidity are perfectly balanced for superior sensitivity.

SPIFE 4000
Auto IFE with SPIFE 4000
Auto SPE with SPIFE 4000
Specifications

Cat. No.	Item
1620	SPIFE 4000, 110V
1621	SPIFE 4000, 220V
1622	SPIFE 4000, 100V/50Hz
1623	SPIFE 4000, 100V/60Hz
1625	SPIFE 4000 Replacement Electrodes
1630	SPIFE 4000 Cassettes (10/pkg)
1635	SPIFE 4000 Rigid Antisera Template
1636	SPIFE 4000 Serum Separator Sleeves, 28/pkg
2304	SPIFE 4000 IFE Antisera Pipette Tips, 96/pkg
2305	SPIFE 4000 IFE Sample Trays, 10/pkg
2306	SPIFE 4000 Antisera Template Treatment
2307	SPIFE 4000 Maintenance Blotters, 20/pkg
2308	SPIFE 4000 Gel Staging Lid
2309	SPIFE 4000 Contact Sheets (5/pkg)
2315	SPIFE 4000 Split Beta SPE Sample Trays, 10/pkg
2319	SPIFE 4000 Applicator Blades
1033	Neutral Density Densitometer Control for SPIFE 4000
9136	Service Manual for SPIFE
1154	Extended Warranty for SPIFE 4000
1156	Consolidated Services for SPIFE 4000

Integrated Sample Handler -

•	Liquid sensing
•	Positive patient ID from tube to final report
•	Automatically makes dilutions for IFE

Auto Applicator and Electrophoresis -

•	Auto blotting and sample application
•	Peltier-driven temperature controlled chamber
•	Auto reagent and antisera application

Integrated Auto Scannings -

•	Automatic setting of scan parameters
•	Full editing and interpretation, if the operator chooses
•	Final results can be printed and/or exported to LIS

Auto IFE with SPIFE 4000

SPIFE 4000 automatically performs all the steps for immunofixation
Auto Dilutions – the sample handler performs all the needed dilutions and applies samples to gels
Auto Antisera –- Antisera are automatically applied to gels. Affinity and avidity of the antisera are balanced for best sensitivity with the least amount of blush.
Auto Press and Blot – Assures clear backgrounds, before moving into the auto stainer
Throughput – Four profiles per gel; up to 100 IFEs per 8 hours

Cat. No.
2300	- 40 tests
2301	- 400 tests

Auto SPE with SPIFE 4000

No protein retention – application system is unaffected by triglycerides or large proteins
Active cooling –- excellent split-beta separation with no degradation
Great results – resolution and presentation are excellent
Throughput – 28 samples per gel; up to 756 per 8 hours

Cat. No.
2310	- 280 tests
2311	- 2800 tests

Performance Specifications

Tests:
Serum Proteins and IFE

Media Types:
Agarose and cellulose acetate gels

Maximum Sample Size:
5 x 5 in. (12.7 x 12.7 cm)

Input Power:
120 VAC, 60 Hz, 1000 Watts
230 VAC, 50 Hz, 1000 Watts

Leakage Current:
< 3.5 mA. sinusoidal

Dimensions: Height x Width x Depth:
SPIFE 4000 Instrument & Fluids Table:
60 x 79 x 32 (152.4 x 200.7 x 81.3 cm)

Computer, Monitor, and Keyboard:
22 x 18 x 24 in. (56 x 48 x 61 cm)

Scanner:
< or equal to 8 x 13 x 22 in. (20 x 33 x 56 cm)

Printer:
10 x 14 x 14 in. (26 x 36 x 36 cm)

Total Weight: 350 lbs (158.8 kg)

Environment: 15° to 27°C (59° to 80°F)

Instrument Operating Environment:

Ambient Temperature Range -
15° to 27°C (59° to 80°F)

Altitude tested up to 2000 meters

Complete specifications for the scanner, monitor and printer can be found in their respective owner's guide.

Densitometer Specifications

Scanning Parameters:
Gain: Automatic or Manual
Over range: > 100% of full scale
Optics Mode:
Visible Transmittance (white light)

Aperture Sizes (mm):
1....... 1 x .1
2....... 2 x .2
3....... 3 x .3
4....... 4 x .4
5....... 6 x .6
6....... 7 x .7
7....... 8 x .8
8....... 2.5 x .25
9....... 5 x .1
10......5 x .25
11..... 5 x .5
12 .....12 x 12

Scan Length:
Variable from 0.01 to 221.5 mm

Zeroing:
Automatic

Light Source:
Cold Cathode Fluorescent (white light)

Light Detectors:
CCD Array

Optical Density Linearity:
0.05 to 2.3

Operating System:

*Windows is a registered trademark of Microsoft Corporation in the United States and other countries.
Microsoft ® Windows® XP

Electrophoresis Timer Range: 1 sec. to 90 min. in 1 second increments
Chamber Temperature Range: 10° to 30°C
Pre-Drying Temperature Range: 30° to 62°C
Final Drying Temperature Range: 30° to 65°C (86° to 149°F)
Fuses: F1, F2 15A/250V slow blow
Pump System: 2 peristaltic pumps, 1 syringe pump
Test Tubes: 13-16 mm diameter, 75-100 mm height (Minimum sample volume 500uL)

Bar Codes:

Type	Character Density Minimum Bar Width	Ratio Thin to Thick	Maximum Characters Bar Width
Code 3 of 6 (Code 9) (alphanumeric)	0.0075	3:1	10
Codabar (numeric)	0.0098	n/a	10
Interleaved 2 of 5 (numeric)	0.0075	3:1	20
UPC - 12 (numeric)	0.014	n/a	13
EAN, L reduced (numeric)	0.014	n/a	13
CODE 128 (alphanumeric)	0.0075	3:1	25

Spife 4000

Electrophoresis Instrument and LIS Interfacing:

Ports: 3-wire RS232, no hardware handshaking

Programmable Baud Rate:	1200, 2400, 4800, 9600, 14400, 19200
Programmable Data Bits:	7, 8
Programmable Stop Bits:	1, 2
Programmable Parity:	None, Odd, Even
Programmable Protocol:	None, XON-XOFF, ASTM

Auto Immunofixation

• Color-coded antisera stored on-board, ready to use

• Antisera applied through rigid template to eliminate crossover

• Auto press and blot to assure clear background

Auto Staining / Drying

• On-board staining with no gel transfers

• Destain and wash for clear backgrounds

• Automated drying and transfer to scanner

Integrated Auto Scan

spife4000 reports can be printed or exported to LIS

• Automatic setting of scan parameters

• Full editing and interpretation capabilities

• Final results can be printed and/or exported to LIS

SPIFE 4000 Brochure