SPIFE Protein Electrophoresis

SPIFE Proteins

Split-Beta or 5-Band SPE

While some laboratorians prefer classic serum protein separations, others prefer a split-beta separation. With SPIFE Split-Beta gels, the proteins migrating in the beta region are split into two fractions, Beta 1 and Beta 2, between transferrin and C3 complement. In some instances, this may allow easier detection of small beta-migrating monoclonal gammopathies. Both the classic and split-beta formulations provide crisp, clear separation of the protein fractions.

●	Samples are applied neat, no dilution hassles
●	SPIFE’s application system is unaffected by triglycerides or
	large proteins
●	Throughput of 1,400 samples in one 8-hour shift
●	New QuickGel 10/20 sample gels plus SPIFE 20, 40, 60, 80 &
	100 sample gels to suit any testing volume

Classic Serum Protein Kits
Cat. No.	Item	Description
3460	SPIFE SPE-60 Kit (600 tests)	10 x 60 samples
3461	SPIFE SPE-40 Kit (400 tests)	10 x 40 samples
3462	SPIFE SPE-20 Kit (200 tests)	10 x 20 samples

Kits include: 10 Gels, Acid Blue Stain, Citric Acid Destain, Blotters, Modified Applicator Blades.

Classic Urine and CSF Protein Kits
Cat. No.	Item	Description
3460U	SPIFE SPE-60 Kit for Urines	10 x 60 samples
3461U	SPIFE SPE-40 Kit for Urines	10 x 40 samples
3462U	SPIFE SPE-20 Kit for Urines	10 x 20 samples

Kits include: 10 Gels, Acid Blue Stain, Citric Acid Destain, Blotters, Modified Applicator Blades.

Split Beta Serum Protein Kits
Cat. No.	Item	Description
3398	SPIFE Split Beta SPE-100 Kit	10 x 100 samples
3399	SPIFE Split Beta SPE-80 Kit	10 x 80 samples
3420	SPIFE Split Beta SPE-60 Kit	10 x 60 samples
3421	SPIFE Split Beta SPE-40 Kit	10 x 40 samples
3422	SPIFE Split Beta SPE-20 Kit	10 x 20 samples

Kits include: 10 Gels, Acid Blue Stain, Citric Acid Destain, Blotters, Modified Applicator Blades.

Split Beta Urine and CSF Protein Kits
Cat. No.	Item	Description
3398U	SPIFE Split Beta SPE-100 Kit for Urines	10 x 100 samples
3399U	SPIFE Split Beta SPE-80 Kit for Urines	10 x 80 samples
3420U	SPIFE Split Beta SPE-60 Kit for Urines	10 x 60 samples
3421U	SPIFE Split Beta SPE-40 Kit for Urines	10 x 40 samples
3422U	SPIFE Split Beta SPE-20 Kit for Urines	10 x 20 samples

Kits include: 10 Gels, Acid Blue Stain, Citric Acid Destain, Blotters, Modified Applicator Blades.

Additional Supplies and Equipment for QuickGel Split Beta and SPE SPIFE Procedures:
Applicator Blade Weights (Cat. No. 3387), QuickGel Gel Block Remover (Cat. No. 1262), SPE Normal (Cat. No. 3424), SPE Abnormal (Cat. No. 3425), REP Prep (Cat. No. 3100), QuickGel Dispo Cup Tray (Cat. No. 3353), SPIFE QuickGel Electrode (Cat. No. 1111), SPIFE QuickGel Holder (Cat. No. 3358), SPIFE QuickGel Chamber Alignment Guide (Part No. 86541003)

QuickGel Split Beta SPE Kit for SPIFE
Cat. No.	Item	Description
3350	QuickGel Split Beta SPE Kit	10 x 10/20 samples*
3405	QuickGel SPE Kit for SPIFE (5-Band)	10 x 10/20 samples*

Kits include: 10 QuickGel Gels, Acid Blue Stain, QuickGel Blotters, Citric Acid Destain, 10 Modified Applicator Blades for use with serum samples.
* Additional Blades (Cat No 3451) and Sample Cups (Cat No 3460) can be purchased to run up to 20 serum samples per gel. For CSF and urines, SPIFE Applicator Blades (Cat No 3450) and Shallow Well Sample Cups (Cat No 3369) must be used or the samples applied using a QuickGel template (Cat No3426).

Serum Protein Controls

Cat. No.	Item
3424	SPE Normal Control
3425	SPE Abnormal Control

These lyophilized controls are prepared from pooled human serum and are for use with the SPIFE SPE Vis and Split-Beta SPE kits listed above. Reconstituted controls are stable 5 days stored at 2 to 8° C. Each control is assayed for total protein, albumin, alpha1, alpha2, beta and gamma globulins.