AggRAM™

AggRAM

Cat. No.	Item
1484	AggRAM Analyzer
1486	AggRAM Module

The AggRAM, for platelet aggregation and ristocetin cofactor testing offers unsurpassed precision, ease of use and flexibility. AggRAM couples four-channel laser optic modules with a powerful Windows interface. Up to two modules can be run per Remote Analyzer computer, providing up to eight channels to handle your workload.

Advanced Laser Optics
Unlike the tungsten lamp optics of old, the AggRAM uses a laser diode to provide beautiful precision across all channels. Upon start-up, AggRAM performs a laser balance check to ensure reproducibility day to day, year to year. Advanced optics coupled with an improved algorithm for slope calculation greatly improve precision for ristocetin cofactor testing.

Powerful, User-Friendly Interface
The AggRAM system software takes full advantage of the Windows platform. On-screen prompts step users through testing. The ability to open multiple windows greatly increases efficiency. For instance, watch the channel display in real time in one window while starting a new worklist in another. Open a third window and print previous runs or observe any combination of channels. Additional features include:

● Three levels of password protection, from user to administrator
● Quick access to data input screens
● Barcode and bi-directional LIS capable

Data Handling Features
Automatically calculates slope and max % aggregation; displays and print lag phase; automatically calculates curves and smooths; reference overlay

Customized Reports
Includes data from one channel or four, one patient or the whole run; pulls from data archives to profile patient results over time.

Quality Control
Evaluates and charts QC for ristocetin cofactor based on Westgard rules; generates Levy-Jennings charts, standard curves, etc with an integral action log.

 

 

 

AggRAM Specifications

Test Types: Platelet Aggregation and Ristocetin Cofactor
Absorbance Range: 0.0 to 2.0 O.D.
Measuring Wavelength: 650 nm
Optical Chambers per module: Four chambers for combined or individual measurements
Cuvettes: 8 mm x 60 mm (silicone coated glass)

 

Stir Bars: 3.5 mm x 4 mm (coated magnet)
Incubation and Reaction Temperature: 37°C +/-1°C
Graphs: -20% to 110% activity (+/- 0.5%) versus time
Instrument Operating Environment: Ambient Temperature Range 15° to 30°C (59° to 86°F)
Module:
Input Power: 110/220 Vac, 50/60 Hz, 1200 Watts Maximum
Dimensions: 6" (15.24 cm) Tall x 10" (25.40 cm) Wide x 17" (43.18 cm) Deep
Weight: < 15 lbs (6.75 kg)
Computer:
800 MHz or higher processor (Intel Pentium/Celeron family)
64 MB or higher of RAM
1.5 GB of available hard disk space
Microsoft® Windows® 2000 Operating System
CD-RW drive
Super VGA (800 x 600) or higher resolution video adapter monitor.
Keyboard and Mouse.
Hp Deskjet 6122 Printer.

AggRAM Accessories and Supplies

Cat. No.	Item
1473	Siliconized Cuvettes for AggRAM and PACKS-4, 200/pkg
1479	Scale Set Solutions (650 nm) for Platelet Assays, 1 each Low and High Calibrator
1489	AggRAM Stir Bars, 30/pkg
9139	Service Manual for AggRAM

 

Platelet Aggregation Reagents

Cat. No.	Item
5366	ADP, 2 x 1 mL
5367	Epinephrine, 2 x 1 mL
5368	Collagen, 2 x 1 mL
5369	Platelet Aggregation Kit (5366, 5367, 5368)
5199	Ristocetin (for RIPA), 10 x 0.5 mL
5365	Tris-Buffered Saline, 1 x 125 mL

 

Ristocetin Cofactor Reagents

Cat. No.	Item
5370	Ristocetin Cofactor Kit, 60 tests
5371	Lyophilized Platelets, 5 x 5 mL
5373	Risto Cofactor Abn Control, 5 x 0.5 mL
5372	Ristocetin (10 mg/mL), 5 x 1.5 mL
5365	Tris-buffered Saline, 1 x 125 mL
5356	Lyophilized Platelets, 5 x 10 mL

 

PACKS-4 Accessories and Replacement Parts

Cat. No.	Item
1463	Air Filters for PACKS-4, 3/pkg
1473	Siliconized Cuvettes for AggRAM and PACKS-4, 200/pkg
1474	PACKS-4 Stir Bars, 30/pkg
1481	PACKS-4 Micro Volume Pipettor (5, 10, 25 µL)
1482	PACKS-4 Pipettor (50, 75, 100 µL)
1475	Disposable Pipette Tips (50-200 µL), 1000/pkg
1476	Disposable Pipette Tips (100-1000 µL), 1000/pkg
1477	PACKS-4 Replacement Lamp, 1/pkg
1478	4-Color Printer Cartridge for Citizen printers
1479	Scale Set Solutions (650 nm) for Platelet Assays, 1 each Low and High Calibrator
1480	Scale Set Solutions (405 nm) for Chromogenics, 1 each Low and High Calibrator
1485	Black Printer Cartridge for Citizen printers

Platelet Aggregation Reagents

Platelet Aggregation Kit

Cat. No.	Item
5369	Platelet Aggregation Kit
	ADP Reagent, 2 x 1 mL
	Collagen Reagent, 2 x 1 mL
	Epinephrine Reagent, 2 x 1 mL

The Platelet Aggregation Kit is used to identify normal and abnormal platelet function, to quantitate platelet response and to determine the patient's response to anti-platelet drug therapy. The reagents are optimized for use with the Helena AggRAM analyzer, but may be used on any turbidometric aggregometer of similar quality.

ADP Reagent

Cat. No.	Item
5366	ADP, 2 x 1 mL

ADP is a lyophilized preparation of adenosine-5-diphosphate. ADP is for use in routine platelet aggregation studies for the evaluation of qualitative platelet function disorders. The concentration of the reconstituted ADP reagent is 20 mM.

 

Collagen Reagent

Cat. No.	Item
5368	Collagen, 2 x 1 mL

The collagen reagent is a clear, stabilized liquid reagent prepared from equine tendon, which is one of the world's best collagen preparations. Collagen is used in routine platelet aggregation studies for evaluation of qualitative platelet function disorders. The collagen reagent stock solution is reconstituted to a concentration of 100 mg/mL.

Epinephrine Reagent

Cat. No.	Item
5367	Epinephrine, 2 x 1 mL

Epinephrine is a lyophilized preparation of L-epinephrine bitartrate. Epinephrine is for use in routine platelet aggregation studies for evaluation of qualitative platelet function disorders. After reconstitution, epinephrine concentration is 300 mg.

Arachidonic Acid Reagent

Cat. No.	Item
5364	Arachidonic Acid, 2 x 1 mL

Arachidonic Acid is a lyophilized preparation of sodium arachidonate for use in platelet aggregation studies of abnormal function, especially dysfunction induced by aspirin and aspirin-containing medications.

 

Ristocetin (for Aggregation Assays)

Cat. No.	Item
5199	Ristocetin, 10 x 0.5 mL

This Ristocetin is for use in platelet aggregation studies for confirmation of von Willebrand Disease (vWD) and Bernard-Soulier Syndrome. Normal platelet-rich plasma exhibits a positive aggregation response to the addition of ristocetin using a final concentration of 12-15 mg/mL. The majority of vWD patients exhibit a negative response to ristocetin as do Bernard-Soulier patients.

Helena Ristocetin Cofactor Assay Kit

Cat. No.	Item
5370	Ristocetin Cofactor Kit, 50 tests
	Lyophilized Platelets, 4 x 5.0 mL
	Ristocetin Cofactor Abnormal Control, 2 x 0.5 mL
	S.A.R.P. Assayed Reference Plasma, 2 x 1.0 mL
	Helena Ristocetin - 10 mg/mL, 2 x 1.5 mL
	Tris-Buffered Saline, 1 x 35 mL

Ristocetin cofactor assays measure the ability of von Willebrand factor (Ristocetin cofactor) in the patient's plasma to agglutinate formalinized platelets in the presence of ristocetin. Decreased levels of ristocetin cofactor activity are associated with von Willebrand Disease.

 

Lyophilized Platelets

Cat. No.	Item
5356	Lyophilized Platelets, 5 x 10 mL
5371	Lyophilized Platelets, 5 x  5 mL

Lyophilized Platelets are a standardized suspension of fixed platelets derived from platelet concentrate. Lyophilized Platelets are for use in the quantitation of ristocetin cofactor activity in plasma. The platelets are lyophilized for stability. Tris-Buffered Saline (Cat. No. 5365) should be used for reconstitution. The concentration of the reconstituted platelet suspension is approximately 200,000/mL. Platelets in Cat. No. 5356 show a slower reaction rate.

Ristocetin Cofactor Abnormal Control Plasma

Cat. No.	Item
5373	Ristocetin Cofactor Abnormal Control, 5 x 0.5 mL

The control is to be used in the assay of ristocetin cofactor. It contains citrated human plasma with a level of ristocetin cofactor activity below 50%.

Ristocetin - 10 mg/mL (for Ristocetin Cofactor Assays)

Cat. No.	Item
5372	Ristocetin - 10 mg/mL, 5 x 1.5 mL

The reagent is to be used in the quantitative determination of ristocetin cofactor activity. The ristocetin reagent is prepared from Nocardia lurida and is used in the tests for von Willebrand Disease and Bernard-Soulier Syndrome.

Tris-Buffered Saline

Cat. No.	Item
5365	Tris-Buffered Saline, 1 x 125 mL

Tris-Buffered Saline is used for reconstitution and dilution of some of the reagents used in platelet aggregation assays and ristocetin cofactor assays.